〔Abstract〕 To establish an in vitro culture model of rat corpora cavernous smooth muscle cells（CC- SMCs）using a modified tissue block adherence method. Methods Corpus cavernosum smooth muscle tissue was digested with collagenase type I and subsequently cultured using an adherent method. Cells were purified via differ- ential adhesion and identified through immunofluorescence and Western blotting. Results CCSMCs began to emerge from the tissue block after 3 days，increased significantly by day 7，and converged by day 12. Post- passage，CCSMCs exhibited strong proliferation and a“peak-to-valley ”phenomenon. After purification，the cells tested positive for α-smooth muscle actin (α-SMA），confirming the successful establishment of the in vitro culture model. Conclusion The modified tissue block adherence method is a cost-effective and efficient way to obtain high-purity CCSMCs.