Acta Universitatis Medicinalis Anhui

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Weng Jianping

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Ameliorating effects of tetrahydrocurcumin and its nano-preparations on lipopolysaccharide-induced depression in mice

Tan Hui 1 , Li Yuanping1 , Meng Jingyuan1 , Ma Tengteng1 , Yang Yan1 , Yang Zhengmao2 , Ma Jiaqing1 , Xie Jianping3 , Guo Ying1

DOI: 10 . 19405/j . cnki . issn1000 - 1492 . 2025 . 01 . 011

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abstract:
To investigate the antidepressant effects and the underlying mechanisms of tetrahydrocurcu-min (THC) and its nanoparticle formulation (THCN) . Methods Forty-six male ICR mice were randomly divided into Con group , LPS group , THC group , THCN group and SER group . A mouse depression model was established by intraperitoneal administration of LPS . The anxiety and depression-like behaviors of mice were evaluated by open field test (OFT) and forced swimming test (FST) . Myelin staining was applied to assess the extent of demyelina- tion in the prefrontal cortex of the mice . The prefrontal cortex and hippocampus were further examined for the ex- pression levels of glial fibrillary acidic protein (GFAP) and Toll-like receptor 4 (TLR4) through quantitative im- munofluorescence assays . Results Compared with the Con group , the LPS group showed increased anxiety-like and depressive-like behaviors in both the long-term and short-term experiments (P < 0. 05) ; the degree of demyeli- nation increased in the LPS group of the long-term experiment (P < 0. 01) ; the expression of GFAP was reduced in the LPS group of the short-term experiment (P < 0. 01) , while the expression of TLR4 increased (P < 0. 05) ; the expression of TLR4 decreased in the THC group (P < 0. 01) ; the expression of GFAP in the prefrontal cortex of the THCN group was reduced (P < 0. 01) , while the expression of TLR4 increased ( P < 0. 05) . Compared with the LPS group , the THC group showed reduced depressive-like behaviors in the long-term experiment ( P < 0. 05 ) , while the anxiety-like and depressive-like behaviors of the THCN group and the SER group were reduced ( P < 0. 05) , and the anxiety-like and depressive-like behaviors of the THC group and the THCN group were reduced in the short-term experiment (P < 0. 05) ; the degree of demyelination was reduced in the THC group , THCN group and SER group in the long-term experiment (P < 0. 05) ; the expression of GFAP increased in the THC group of the short-term experiment (P < 0. 05) , while the expression of TLR4 was reduced (P < 0. 05) , and the expression of GFAP increased in the THCN group (P < 0. 05) . Compared with the THC group , the THCN group and the SER group showed reduced anxiety-like behaviors in the long-term experiment (P < 0. 05) ; the expression of GFAP in the prefrontal cortex of the THCN group was reduced in the short-term experiment (P < 0. 05) , while the expres- sion of TLR4 in the hippocampal DG area increased in the short-term experiment (P < 0. 01) . Conclusion Tetra- hydrocurcumin and its nanoparticle formulation both exert significant ameliorative effects on depression-like behav- iors and demyelination in mice induced by lipopolysaccharide . The antidepressant mechanism of THC appears to be mediated through the down-regulation of TLR4 and the up-regulation of GFAP. The mechanism underlying the anti- depressant action of THCN seems predominantly focused on the enhancement of GFAP expression .

Effect of chromosome-instability-associated gene GALNT7 on proliferation and apoptosis of HCT116 colon cancer cells

Fang Xiao1 , 2 , Zhao Wei 1 , 2 , Yu Wenying1 , 2 , Pei Lingjie1 , 2 , Qian Wenxuan1 , 2 , Zhao Ya1 , 2

DOI: 10 . 19405/j . cnki . issn1000 - 1492 . 2025 . 01 . 013

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Protective effect of Rhodiola rosea bionic nanomedicine on pancreas of acute pancreatitis rats and its mechanism

Zhang Tongzhe , Zhao Xirong , Li Peiwu

DOI: 10 . 19405/j . cnki . issn1000 - 1492 . 2025 . 01 . 01

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abstract:
To explore the protective effect and mechanism of Rhodiola rosea( Rho) bionic nanomedi- cine in rats with acute pancreatitis . Methods Erythrocyte membrane vesicles ( EMV) were used as biomimetic nanomedicine coating materials to construct Rho biomimetic nanomedicine . Meanwhile , rat models of acute pancre- atitis were constructed and divided into acute pancreatitis model (AP) group , Rho group , EMV group and Rhodio- la rosea erythrocyte membrane vesicles (R-EMV) group . R-EMV group rats were further treated with NLRP3 acti- vator BMS-986299 (R-EMV + BMS-986299 group) and inhibitor MCC950 (R-EMV + MCC950 group) . The chan- ges of abdominal water volume were observed , and serum levels of interleukin (IL-6) , IL-1β, endothelin (ET) , diamine oxidase (DAO) , malondialdehyde (MDA) , superoxide dismutase (SOD) and glutathione ( GSH) were detected by enzyme-linked immunosorbent assay (ELISA) . Hematoxylin-eosin (HE) staining was used to observe the pathological changes of pancreatic tissue . Immunohistochemistry , reverse transcription-polymerase chain reac- tion (RT-PCR) and Western blot were used to analyze the expression of NOD-like receptor protein 3 (NLRP3) and nuclear transcription factor-Kappa B( NF-κB) in pancreatic tissue . Results The prepared R-EMVs were nearly circular , the average particle size was (244. 61 ±1 . 08) nm , and the Zeta potential was - ( 11 . 13 ±1 . 25) mV . At the same time , the EMV could load (58 . 67 ±0. 79) μg of Rho . Compared with Sham group , the abdominal water volume of rats in AP group was significantly higher ( t = 33 . 79 , P < 0. 01) , and the levels of peripheral blood immune indexes IL-1β, IL-6 , ET and DAO increased ( t = 38 . 25 , 42. 54 , 29. 20 , 34. 92 , all P < 0. 01) . In AP group , there were obvious tissue hyperemia and edema , widening of lobular space , infiltration of a large number of inflammatory cells , and increased pancreatic pathological score ( t = 30. 06 , P < 0. 01) . Compared with AP group , abdominal water volume , dry-wet weight ratio of pancreas , pathological score , amylase and lipase levels in Rho group decreased (F = 1 523 . 7 , 543 . 3 , 839. 9 , 446. 1 , 172. 2 , P < 0. 05) . Compared with other groups , the lev- els of serum IL-1β, IL-6 , ET and DAO in AP group decreased to some extent. Compared with AP group , NF-κB p65 and NLRP3 protein levels and NF-κB and NLRP3 immunohistochemical staining scores decreased in R-EMV group ( t = 24. 54 and 26. 91 , both P < 0. 001) . At the same time , the levels of serum IL-1β, IL-6 , ET , DAO , MDA , SOD and GSH in R-EMV + MCC950 group were significantly lower than those in AP group , R-EMV group and R-EMV + BMS-986299 group . Conclusion R-EMV has a good pancreatic protection effect in acute pancreati- tis , which is related to reducing the activity of NF-κB/NLRP3 pathway and down-regulating the expression of in- flammatory factors such as IL-6 and oxidative stress indicators such as MDA .

Construction and evaluation of a nomogram prediction model for biliary stricture after liver transplantation

Xie Hongyue1 , Xu Xiaoliang2 , Liu Qiaoyu2 , Sun Beicheng1 , 2

DOI: 10 . 19405/j . cnki . issn1000 - 1492 . 2025 . 01 . 022

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abstract:
To explore the risk factors of biliary stricture after liver transplantation and to construct a no- mogram prediction model . Methods The clinical data of 208 liver transplant recipients in hospital were retrospec- tively analyzed , including 54 cases in the biliary stricture group and 154 cases in the non-biliary stricture group . Multivariate Logistic regression analysis was used to screen out independent predictors , fit the prediction model and construct a visual nomogram to evaluate the prediction model . Survival curves were drawn and multivariate Cox re- gression analysis was performed . Results Autoimmune liver diseases (OR = 6. 610 , 95% CI: 1 . 410 - 30. 99) , al- anine aminotransferase (ALT) (OR = 1 . 007 , 95% CI: 1 . 003 - 1 . 011) , warm ischemia time ( OR = 1 . 972 , 95% CI: 1 . 399 - 2. 780) , cold ischemia time (OR = 1 . 016 , 95% CI: 1 . 010 - 1 . 022) , cytomegalovirus infection ( OR = 6. 037 , 95% CI: 1 . 480 - 24. 63) and hepatic vascular stenosis (OR = 7 . 784 , 95% CI: 2. 312 - 26. 20) were in- dependent predictors of biliary stricture after liver transplantation . The area under the curve (AUC) of the nomo- gram prediction model was 0. 921 , the cut-off value was 0. 238 , the sensitivity was 0. 889 , and the specificity was 0. 838 . The model showed good discrimination . The Brier score was 0. 092 , Hosmer-Lemeshow goodness-of-fit test P = 0. 253 , Calibration curve (B = 1 000) was in good agreement , and the model showed good calibration . Deci- sion curve analysis (DCA) showed that the application of the model could benefit liver transplant recipients . The postoperative follow-up time was 27 - 60 months . The cumulative survival rate of the non-biliary stricture group was better than that of the biliary stricture group (P = 0. 019) , but multivariate Cox regression analysis showed that bili- ary stricture ( HR = 1 . 194 , 95% CI: 0. 624 - 2. 285) was not an independent risk factor for survival after liver transplantation . Conclusion The nomogram model based on autoimmune liver diseases , ALT , warm ischemia time , cold ischemia time , cytomegalovirus infection and hepatic vascular stenosis performs well and can be used to predict the occurrence of biliary stricture after liver transplantation .

The role and mechanism of DA/GSK-3 signaling in manic-like behavior induced by acute sleep deprivation in mice

Cui Yangfeng , Chen Ming

DOI: 10 . 19405/j . cnki . issn1000 - 1492 . 2025 . 01 . 012

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abstract:
To explore the effects of acute sleep deprivation on manic-like behavior in mice and its pos- sible molecular mechanisms . Methods Seventy-three 8-week male C57BL/6 mice were randomly divided into two groups : control group ( ctrl group , n = 36) and acute sleep deprivation group ( SD group , n = 37) . After acute sleep deprivation , the motor and exploration ability of mice were evaluated by open field test and elevated plus maze . Depression-like behavior of mice was evaluated by tail suspension test. Neuronal activation in brain tissue was observed by c-fos staining , neurotransmitter levels in hippocampus ( HIP) and medial prefrontal cortex ( mPFC) were detected by ELISA Kit , and glycogen synthase kinase-3 β(GSK-3 β) and phosphorylated GSK-3 β(p- GSK-3 β) levels in HIP and mPFC were detected by Western blot. Results Compared with the ctrl group , the to- tal movement distance (P < 0. 05) and the time in the central area (P < 0. 05) of the mice in SD group significant- ly increased in the open field test , and the open arm time (P < 0. 000 1) and open arm distance (P < 0. 01) sig- nificantly increased in the elevated plus maze . In the tail suspension test , the immobility time (P < 0. 01) was sig- nificantly reduced . In SD group , the expression of c-fos in HIP and mPFC increased (P < 0. 01) , and the level of neurotransmitter DA in HIP ( P < 0. 05 ) and mPFC ( P < 0. 01 ) significantly increased . Western blot results showed that compared with ctrl group , the ratio of p-GSK-3 βand GSK-3 βin mPFC and HIP significantly decreased in SD group (P < 0. 000 1 , P < 0. 05) , while the level of p-GSK-3 βprotein significantly decreased in mPFC (P < 0. 05) , but there was no significant change in HIP. Conclusion Acute sleep deprivation may regulate the level of GSK-3 through DA in mPFC of mice , inhibiting the serine phosphorylation of GSK-3 and eventually leading to ma- nic-like behavior in mice .

Effect of targeted silencing of DNMT3A on collagen deposition , proliferation and migration activity of mouse lung fibroblasts

Wang Xianchen1 , You Junbo1 , Ling Hui 1 , Fan Jiahao1 , Chen Qi2 , Tao Hui2 , Sha Jiming1

DOI: 10 . 19405/j . cnki . issn1000 - 1492 . 2025 . 01 . 009

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abstract:
To investigate the effect of targeted silencing of DNA methyltransferase 3A (DNMT3A) on collagen deposition , proliferation and migration activity of mouse lung fibroblasts (PFs) . Methods In order to en- sure the proliferation and migration activity of primary fibroblasts , the lung tissues of neonatal C57 suckling mice were taken , PFs were extracted after being sheared , and the morphology was observed and identified under the mi- croscope . PFs cells were activated by 5 ng/ml TGF-β1 for 24 h after cell attachment , and DNMT3A silencing model was constructed by small interfering RNA; The experiment was divided into control group , TGF-β1 group , TGF-β1 + siRNA-NC group and TGF-β1 + siRNA-DNMT3A group . The protein expressions of DNMT3A , α-smooth mus- cle actin ( α-SMA) and Collagen Ⅰ were detected by Western blot; Real time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was used to detect the mRNA expression changes of DNMT3A , α-SMA and Collagen Ⅰ. The proliferation ability of PFs was detected by CCK-8 and EdU staining; the migration ability of PFs was detected by scratch test and Transwell migration test. Results Compared with the control group , TGF-β1 in- duced the increase of DNMT3A in the activated PFs cell group (P < 0. 01) , the protein and mRNA levels of fibro- sis and proliferation related indicators α-SMA and Collagen Ⅰ also increased (all P < 0. 05) , and the proliferation and migration ability of PFs increased (all P < 0. 000 1) . Compared with the siRNA-NC group , the protein expres- sion levels of DNMT3A (P < 0. 000 1) and related indicators α-SMA (P < 0. 01) and Collagen Ⅰ (P < 0. 01) sig- nificantly decreased in the DNMT3A silencing group by Western blot , and the mRNA levels of DNMT3A , α-SMA and Collagen Ⅰby RT-qPCR also decreased (all P < 0. 001) , and the proliferation (P < 0. 01) and migration abil- ity (P < 0. 05) of PFs cells decreased compared with the control group . Conclusion Silencing DNMT3A can in- hibit the deposition of collagen and the proliferation of PFs . DNMT3A can promote the proliferation and migration of PFs , and then promote the activation of PFs and the development of pulmonary fibrosis . This process may be regu- lated by DNA methylation modification .

Effects of resveratrol on cGAS-STING signaling pathway in fibroblast-like synoviocytes of patients with rheumatoid arthritis

Wang Taorong1 , Shao Yubao1 , Liu Nannan2 , Li Wenhao3 , Li Meng4 , Chen Xiaoyu1 , 2

DOI: 10.19405/j.cnki.issn1000-1492.2025.01.010

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abstract:
To investigate the effects of resveratrol (Res) on fibroblast-like synoviocytes (FLS) in pa- tients with rheumatoid arthritis (RA) , and to explore the possible mechanism of Res inhibiting the release of in- flammatory factors from FLS . Methods FLS from RA patients were cultured in vitro and treated with different con- centrations of Res (0 , 20 , 40 , 80 , 160 , 320 μmol/L) . The viability of FLS cells was detected by CCK-8 assay after 12 and 24 h . The contents of inflammatory factor interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) in cell supernatant were detected by ELISA . The expression levels of cyclic guanosine monophosphate- adenosine monophosphate synthase (cGAS) and stimulator of interferon gene (STING) were measured by Western blot; After lentivirus infection with FLS caused the cells to overexpress cGAS , the cells were divided into Control group (blank control) , cGAS group (cGAS overexpression) , Res + cGAS group (Res 160 μmol/L + cGAS overexpression) and Res group (Res 160 μmol/L) . The expression level of STING protein in cells of each group was determined by Western blot , the viability of FLS cells in each group was detected by CCK-8 , and the contents of inflammatory fac- tor IL-6 and TNF-αin the supernatant of cells of each group were detected by ELISA method . Results The results of CCK-8 experiment showed that under 40 , 80 , 160 μmol/L Res treatment , FLS viability decreased significantly after 24 h compared with blank control group ( P < 0. 01) . ELISA results showed that the contents of IL-6 and TNF-αin cell supernatant were also significantly decreased after treatment with Res of 40 , 80 and 160 μmol/L (P < 0. 01) . Meanwhile , Western blot results showed that Res could significantly decrease the protein expression lev- els of STING and cGAS in FLS cells after treatment of 40 , 80 and 160 μmol/L (P < 0. 05 , P < 0. 01) . Compared with the Control group , the expression level of STING protein in FLS increased after overexpression of cGAS (P < 0. 05) ; compared with the Res group , the content of inflammatory factors in the supernatant of FLS and the expres- sion level of STING protein in FLS significantly increased after overexpression of cGAS ( P < 0. 01 , P < 0. 05 ) . Conclusion The appropriate concentration of Res can inhibit the release of inflammatory cytokines in FLS cells , which may be related to the blocking of cGAS-STING signaling pathway .

The outcome of HR-HPV infection and its relationship with cervical cytology in 478 patients with normal cervix in Hefei area

Li Qing , Wang Qingyuan , Zhang Wanying , Wang Wenyan

DOI: 10.19405/j.cnki.issn1000-1492.2025.01.025

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abstract:
To investigate the factors affecting the outcome of high-risk human papillomavirus ( HR- HPV) infection in patients with normal cervix examined by colposcopy in Hefei area and the relationship between persistent HR-HPV infection and cervical cytology . Methods Data of colposcopy patients were collected from 478 HR-HPV infected patients with normal cervix through colposcopy . Their age , number of sexual partners , contracep- tive methods and other relevant basic information were recorded . Vaginal interferon use , HR-HPV infection at year 1 and year 2 , and cervical liquid-based cytology test (LCT) results were tracked , univariate and multivariate ana- lyses were performed based on basic information , and ROC curves were plotted . Results The HR-HPV clearance rate at 1 year was 59. 41% , and the clearance rate at 2 years was 66. 75% . The other 12 types of infection (31 , 33 , 35 , 39 , 45 , 51 , 52 , 56 , 58 , 59 , 66 , 68) were more common than the 16 and 18 types . Univariate and mult- ivariate analyses showed that age > 50 years , number of sexual partners ≥2 , and history of cervical conectomy in- creased the risk of persistent HR-HPV infection ( χge = 21 . 676 , P < 0. 001 ; χumber of sexual partners = 8. 262 , P = 0. 004 ; χistory of cervical conectomy = 11 . 267 , P = 0. 001) . The risk of HR-HPV infection was significantly lower when condom or vaginal interferon was used ( χondom use = 10. 885 , P = 0. 001 ; χnterferon use = 4. 099 , P = 0. 043) . The area under the ROC curve (AUC) of combined diagnosis of HR-HPV persistent infection was higher than that of single diagnosis , and the AUC of combined diagnosis was 0. 737 . Persistent HR-HPV infection was an independent risk factor for abnormal LCT , and the AUC predicted by the model was 0. 755 . No cancer was found in patients with persistent HR-HPV infection for 2 years , and the proportion of abnormal LCT was higher than that in patients with negative HR-HPV . The difference was statistically significant ( χ2 = 39. 64 , P < 0. 001) . Conclusion The com- bined ROC model constructed for patients > 50 years old , with multiple sexual partners , history of cervical surgery , no vaginal interferon use , and no condom use has certain value in predicting persistent HR-HPV infection , and per- sistent HR-HPV infection has predictive value in predicting LCT abnormalities .

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