Acta Universitatis Medicinalis Anhui

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Anhui Provincial Education Department

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Anhui Medical University

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Weng Jianping

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ISSN 1000 - 1492

CN 34 - 1065/R

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The correlation between uteroglobin-related protein 1 and primary hypothyroidism

Lu Chenyang , Ma Xingran , Xu Tian , Zuo Chunlin

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.021

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abstract:
Abstract Objective To explore the correlation between uteroglobulin-related protein 1 ( UGRP1) and primary hypothyroidism. Methods Ninety-six patients with primary hypothyroidism were selected , including 66 patients with positive thyroid peroxidase antibodies (TPOAb) or anti-thyroglobulin antibodies (ATG) as the antibody-posi- tive group , 30 patients with negative thyroid autoantibodies as the antibody-negative group , and 96 healthy people as the control group . The general clinical data , thyroid-related indicators and serum UGRP1 levels were compared among these three groups . Human thyroid normal cells (NTHY-ORI 3-1) were transfected with plasmids in vitro , thus establishing the control group as well as the UGRP1 group . Enzyme linked immunosorbent assay (ELISA) was used to detect and compare the T4 level in the cell culture supernatant. Results The differences in thyroid stimu- lating hormone (TSH) , TPOAb and ATG among the three groups were statistically significant ( P < 0. 05) . The serum UGRP1 levels in the antibody-positive (303 . 97 ±156. 00) pg/m Land antibody-negative groups (352. 13 ± 188. 37)pg/m L were higher than those in the control group(237 . 54 ±137 . 20)pg/m L，and the differences between the groups were statistically significant ( P = 0. 005) . Meanwhile , there was no statistically significant difference between the antibody-positive and antibody-negative groups . Multi-factor Logistic regression analysis showed that UGRP1 was the risk factor for the occurrence of primary hypothyroidism (OR = 1 . 004 , 95% CI:1 . 001 - 1 . 007 , P = 0. 007) . The difference between the control group and UGRP1 group in T4 concentration secreted by human thyroid normal cells was not statistically significant. Conclusion Serum UGRP1 levels increase in patients with primary hypothyroidism , and the high expression of UGRP1 may have no direct relation to the function of thyroid cells secre- ting T4 .

Value of decreased carbohydrate antigen 19-9 kinetics for patients with advanced biliary or pancreatic cancers

Zhang Yiyin , Dai Ying , He Ziran , Qu Ziting , Lu Lili , Zhu Qingbo , Qi Xiaowen , Gu Kangsheng

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.018

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Progress in the treatment of Alzheimer′sdisease by Chinese medicine extracts based on C. elegans model

Pei Yuqing1 , Xu Chunyu1 , Shao Xindi 1 , Zhu Yujie1 , Zhou Siyue1 , Zheng Zhiyi 1 , Cheng Fei 1 , Shi Xuan1 , Chen Zhangyue2 , Li Hualing1 , 3

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.026

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A study of deep-learning image reconstruction algorithm in virtual un-enhanced scanning of aortic CTA

Zhang Tianyu , Zhao Xiaoying , Song Jian , Shen Yi , Wu Xingwang

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.022

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abstract:
Abstract Objective To evaluate the clinical value of combining low-dose energy spectrum CT with virtual un-en- hanced (VUE) scanning and deep-learning image reconstruction (DLIR) in aortic CT angiography (CTA) . Meth- ods In a prospective study , 94 patients scheduled for aortic CTA were randomized into two groups : a low-dose en- ergy spectrum group and a standard 100 kVp enhancement group , with 47 patients in each . All patients initially underwent a true un-enhanced (TUE) scan at 120 kVp using adaptive statistical iterative reconstruction-V (ASIR- V) at 40% for image reconstruction . The low-dose group received enhanced scans using gemstone spectral imaging (GSI) mode with DLIR-H , producing 60 keV virtual monoenergetic images (VMIs) and VUE images . The stand- ard group was scanned at 100 kVp , with images reconstructed using ASIR-V at 50% . Parameters were measured including CT values , noise (SD) , signal-to-noise ratio (SNR) , and contrast-to-noise ratio (CNR) for key vascu- lar and muscular areas , alongside the effective radiation dose (ED) . Two radiologists evaluated the image quality using a 5-point scale . Results The low-dose group exhibited significantly higher SNR and CNR values in the as- cending aorta , descending aorta , abdominal aorta , and common iliac artery compared to the standard group (P < 0. 05) , with comparable subjective quality scores . The VUE images also demonstrated superior SNR values in the abdominal aorta , common iliac artery , and psoas major muscle , and CNR value in the ascending aorta compared to TUE images , with similar subjective quality. Importantly , the ED in the low-dose group was about 40% lower than that of the standard group . Conclusion Low-dose energy spectrum CT with DLIR in aortic CTA can significantly enhance SNR and CNR , while approximating the image quality of traditional TUE scans , thereby substantially re- ducing radiation exposure .

Clinical characteristics and drug resistance monitoring of Streptococcus mitis group bloodstream infection

Li Yajuan , Liu Tingting , Huang Ying , Xu Yuanhong

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.020

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abstract:
Abstract Objective To assess the epidemiological , drug resistance , and their relative risk and prevalence for yielding clinical diseases of Streptococcus mitis group (SMG) bloodstream infections in recent years . Methods A total of 50 blood culture specimens were collected from patients with SMG bloodstream infection . These SMG iso- lates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry ( MALDI-TOF MS) . The susceptibility to antibiotics was tested by minimal inhibitory concentrations and Kirby-Bauer (K-B) disk diffusion methods . The data were comprehensively analyzed by statistical software combined with clinical data. Re- sults Five strains were identified in SMG bloodstream infection by mass spectrometry , namely Streptococcus oralis/ mitis (S. oralis/mitis) , Streptococcus pneumonia (S. pneumonia) , Streptococcus gordonii (S. gordonii) , Streptococ– cus sanguinis (S. sanguinis) , and Streptococcus parasanguinis. These SMG showed high resistance to erythromycin and clindamycin , but low resistance to penicillin , ampicillin and ceftriaxone . Reduced hemoglobin and albumin , elevated C-reactive protein and procalcitonin were the common hematological changes in patients with SMG blood- stream infections . In SMG bacteremia , S. gordonii , S. sanguinis and S. orals/mitis were the leading group causing infective endocarditis . Patients with myocardial disease factor were more likely to cause infective endocarditis by S. gordonii and S. sanguinis , compared with S. orals/mitis. S. oralis/mitis bacteremia more occurred in patients with renal transplants progressing to pulmonary infection . Conclusion In this area , β- lactam antibiotics are the best choice for treating SMG. SMG species with closely related phylogenetically show different prevalence and risk of clinical disease in bloodstream infection patients . Early prevention and diagnosis of bacteremia caused by SMG are necessary for clinical diagnosis , treatment and effective control of infectious diseases progression .

Research on the prediction model of agitated symptoms in adolescents with depressive disorders

Zhao Xin1 , 2 , 3 , Liu Lewei 1 , 2 , 3 , Hao Mingru1 , 2 , 3 , Fan Haojie1 , 2 , 3 , Xia Lei2 , 3 , Geng Feng4 , Mo Daming5 , Liu Huanzhong2 , 3 , 6

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.023

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Study on the synergistic effects and mechanisms of IL-25 and house dust mite in promoting eosinophilic asthma allergy

Xu Lina , He Xiaoshuang , Liu Dong

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.009

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abstract:
Abstract Objective To explore the central role of interleukin (IL)-25 in allergy of eosinophil asthma. Methods Forty 4 - 5 week-old C57BL/6 mice were used in this study ( n = 10) . Mice were randomly divided into 4 groups : WT + shRNA NT group , WT + HDM group , IL-25 shRNA + IL-25 group , and IL-25 shRNA + IL-25 + HDM group . According to each group ′s treatment requirement , mice were treated with house dust mite and/or IL- 25 by intranasal infusion . HE staining and PAS staining were used for lung tissue section staining and pathological analysis . The levels of type 2 cytokines in bronchoalveolar lavage fluid (BALF) were determined by ELISA . Flow cytometry (FCM) was used to determine the number of type 2 helper T (Th2) cells , the group 2 innate lymphoid cells (ILC2) and eosinophils in lung tissues . Mice were treated with FITC-labeled DQ-ovalbumin (DQ-OVA) by intranasal infusion to determine the antigen presentation ability of eosinophils infiltrated in the lungs . Results HE staining and PAS staining results showed that , compared with WT + shRNA NT group , a large amount of mucus and a large number of eosinophil infiltration were found in the lungs , and subcutaneous thickening of inflammatory air- way were observed in the pulmonary vessels , alveolar ducts and the whole alveoli in IL-25 shRNA + IL-25 + HDM group . In the IL-25 shRNA + IL-25 group , only a small amount of mucus and a small amount of eosinophil infiltra- tion were found in the lungs , and a small amount of subcutaneous thickening of inflammatory airway were observed in pulmonary vessels , alveolar ducts and the whole alveoli . There was no significant difference in WT + HDM group . Compared with WT + shRNA NT group , the levels of IL-25 , IL-4 , IL-5 , IL-13 , IL-33 and interferon-γ (IFN-γ) in BALF significantly increased; the levels of infiltrated Th2 , ILC2 and eosinophils in the lung signifi- cantly increased; the fluorescence levels of FITC-labeled DQ-OVA in eosinophils infiltrated in lungs significantly increased in IL-25 shRNA + IL-25 + HDM group (P < 0. 05) . IL-25 shRNA + IL-25 group mice also showed an in- crease in the above measurements , but the amplitude were limited (P < 0. 05) . WT + HDM group had no signifi- cant difference . Conclusion IL-25 plays an essential role in the allergy and eosinophil antigen presentation processes in eosinophil asthma mice .

AdipoRon improves fibrosis liver function by regulating lipid metabolisms and remodeling macrophages polarization

Wang Haikun , Yao Ping , Yang Tao , Xi Lili

DOI: 10.19405/j.cnki.issn1000-1492.2025.04.010

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abstract:
Abstract Objective To investigate the role of AdipoRon , an adiponectin receptor agonist , in treatment of carbon tetrachloride (CCl4 ) induced liver fibrosis mice model and the mechanisms . Methods Forty mice were randomly divided into control group , model group , L- AdipoRon group and H-AdipoRon group , with 10 mice in each group . Hepatic fibrosis was induced by intraperitoneal injection of CCl4 solution . The mice in L- and H-AdipoRon groups were given 100 mg/kg and 200 mg/kg AdipoRon by gavage , respectively . The activities of serum aspartate amin- otransferase (AST) and alanine aminotransferase (ALT) were detected by biochemical method . Liver histopatho- logical changes and fibrosis were detected by HE staining , Masson staining and Sirius scarlet stain . The protein ex- pression levels of Collagen I , α-smooth muscle actin ( α-SMA) , matrix metalloproteinase 1 (MMP-1) and matrix metalloproteinase inhibitor 1 (TIMP-1) in mice liver were detected by Western blot. Lipid deposition in liver were detected by oil red O staining. The percentage (% ) of CD68 + iNOS + positive M1-type macrophages in the liver were detected by immunofluorescence . The expression levels of fatty acid synthetase (Fasn) , stearoyl-CoA desatu- rase 1 (Scd1) , fatty acid transporter (Cd36) , peroxissome proliferator activated receptor-α(Pparα) and carnitine palmitoyl transferase 1 α (Cpt1α) in mice liver tissues , as well as M1 macrophage-related genes interleukin-6 (IL- 6) and tumor necrosis factor-α(TNF-α) and M2 macrophage-related genes arginase 1 ( Arg1) , Chil3 chitinase- like 3 (Ym-1) were detected by RT-qPCR assay . Results Compared with model group , in low-dose AdipoRon group and high-dose AdipoRon group , serum ALT and AST activities significantly decreased (P < 0. 05) ; liver tis- sues structure were damaged , liver cells degeneration and inflammatory cells infiltration were improved; collagen fi- ber deposition was also significantly reduced; the relative expression levels of Collagen I , α-SMA and TIMP-1 pro- teins were significantly down-regulated (P < 0. 05) , while the relative expression levels of MMP-1 protein were sig- nificantly up-regulated (P < 0. 05) ; the lipid droplets deposition in livers were significantly reduced . The relative Fasn , Scd1 and Cd36 mRNA expression levels in liver tissues were significantly down-regulated (P < 0. 05) , and the relative Pparαand Cpt1 αmRNA expression levels were significantly up-regulated (P < 0. 05) ; the percentage (% ) of CD68 + iNOS + positive M1-type macrophages significantly decreased (P < 0. 05) ; the relative IL-6 and TNF-αmRNA expression levels significantly decreased (P < 0. 05) , the relative Arg1 and Ym-1 mRNA expression levels were significantly up-regulated ( P < 0. 05 ) . In addition , the improvement effects of high-dose AdipoRon group were better than those of low-dose AdipoRon group ( P < 0. 05) . Conclusion AdipoRon can improve the disorder of lipid metabolisms , inhibit the M1 type macrophages polarization , and improve the liver fibrosis in CCl4 - induced liver fibrosis mice model .

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