〔Abstract〕 Objective To study the antibacterial properties of nano-silver-containing polyelectrolyte multilayer films assembled with heparan sulfate(HEP) and chitosan(CHI) by layer-by-layer self-assembly technology(LBL) on porous titanium surface. Methods Negatively charged HEP and positively charged CHI were alternately adsorbed by LBL to form polyelectrolyte multilayer films, which were deposited on the surface of porous titanium, and then silver ions were loaded on the multilayer films under alkaline conditions. The surface was evaluated by scanning electron microscopy, X-ray photoelectron spectrometer, a measuring instrument for contact angle and inductively coupled plasma emission spectrometer. The antibacterial effect was evaluated by inhibition zone, turbidity method, live/dead bacterial staining and coated plate method. Results Material characterization methods showed polyelectrolyte multilayer films were successfully deposited on the surface of titanium, and nano-silver particles were loaded. Antibacterial experiments showed that the silver-loaded modification significantly improved the antibacterial ability of the coating. Conclusion Silver-loaded HEP-CHI polyelectrolyte multilayer films are deposited on the surface of modified titanium by LBL, which can inhibit the adhesion of bacteria.