〔Abstract〕 Objective To detect the changes of autophagic flux at different stages after oxygen-glucose deprivation-reoxygenation(OGD/R) with several highly sensitive methods. Methods Primary cortical neurons after oxygen deprivation of sugar after reoxygenation(OGD/R) were divided into the experimental OGD/R group and OGD/R+bafilomycinA1(BafA1) group, using an RFP-GFP series fluorescent tags LC3 gene transfection detection cytolysosome and fusion of lysosomes, transmission electron microscope(TEM) observation the ultrastructure of autophagy, p62/SQSTM1 combining LC3 protein to flip the experimental testing p62 and LC3 protein quantitative, p62 immune staining observing the distribution and content. Results Under fluorescence microscope, the ratio of autophagy lysosome to autophagosome increased significantly in OGD/R group, and the changes of autophagy structure in different stages could be observed in TEM. The ratio of soluble p62 and LC3Ⅱ/Ⅰ reflected the activation of autophagic flux, and p62 was mainly distributed in BafA1 group after fluorescence staining. Conclusion Each method has its own advantages, and different methods and indicators can accurately reflect the specific changes of autophagic flux in different stages after neuronal OGD/R. Mastering and applying these methods can effectively explore central nervous system diseases from the perspective of autophagy.