〔Abstract〕 Objective To explore the regulatory mechanism of the long noncoding FGD5-AS1(LncRNA FGD5-AS1) on hepatocellular carcinoma(HCC)progression. Methods The expression level of FGD5-AS1 was measured in tumor tissues and cell lines by RT-qPCR. CCK-8, EdU, flow cytometry, wound healing and transwell chamber assays were performed to investigate the role of FGD5-AS1 in HCC cell proliferation, apoptosis, migration and invasionin vitro. At the molecular level, dual luciferase reporter and RNA pull down assays were performed to identify the interaction among FGD5-AS1, miR-873-5 p and GTPBP4.Results FGD5-AS1 was upregulated in HCC tissues and cells. Moreover, FGD5-AS1 knockdown suppressed HCC cell proliferation, migration and invasion, and induced apoptosisin vitro. Mechanistically, FGD5-AS1 directly bound to miR-873-5 p and competitively inhibit its expression to increase the expression level of GTPBP4 in HCC cells. Finally, our findings indicated that the role of FGD5-AS1 was mediated by miR-873-5 p GTPBP4 axis.Conclusion FGD5-AS1 promotes the proliferation, migration and invasion of HCC cells and inhibits apoptosis by regulating the miR-873-5 p/GTPBP4 axis.