〔Abstract〕 Objective To study the expression and clinical significance of annexin A1(ANXA1) in papillary thyroid carcinoma(PTC) and explore the role of ANXA1 in the development of PTC and its relative molecular mechanism. Methods From the TCGA public database, the relevant data of ANXA1 expression was obtained and analyzed in thyroid cancer and adjacent normal tissues, and explored the relationship between the ANXA1 expression and the patient′s overall survival; then the paraffin sections were collected which contained PTC, nodular goiter(NG), and normal thyroid tissue(NT), and the expression of ANXA1 protein were detected by immunohistochemical method; the fresh tissues were collected and ANXA1 mRNA was checked by the way of qRT-PCR; then the relationship between the expression level of ANXA1 and clinicopathological characteristics was studied. Results Through database analysis, the expression of ANXA1 in thyroid cancer was more prominent than that in NT(P<0.05), and the expression level of ANXA1 was associated with the survival of patients(P<0.05); ANXA1 protein had a higher level in the paraffin sections of PTC, which compared with NT and NG; and ANXA1 mRNA was lower than them. The results of clinical data analysis showed that ANXA1 was related to the location, diameter and lymph node metastasis(P<0.05), but had nothing with the patient′s age, gender and clinical stage. Conclusion ANXA1 has a closed relationship with PTC, which is essential to potential diagnostic and prognostic indicators.