〔Abstract〕 Objective To analyze the results of the micro lymphotoxin cross-matching test and explore the primary distribution of donors and recipients and its clinical significance. Methods 344 patients were analyzed, including age, gender, blood group distribution, donor-recipient relationship, and the influence was also investigated, respectively. Results In the aspect of recipients: the number of male recipients was more than that of female recipients, and the age of recipients was mainly between 21 and 50 years old. The PDL of 31～40 years receivers was the lowest in the low-risk group, which was(3.85 ±2.08); the recipients′ blood types were mostly typed A and O, of which 85.17% of the donors and recipients had the same blood type. In the aspect of donors: 72.67% of the donors were DCD;in the low-risk group, the PDL of the DCD group was(5.30±2.24), which was higher than that of LDR group, and the difference was statistically significant(P<0.05). In LDR, female donors were more than male donors, the donors over 50 years old were the most, type O donors were the most, and 93.02% of the donors were parents. Conclusion Although the number of DCD was more than that of LDR, the positive rate of PDL was lower in LDR, and it can provide kidney sources more quickly and effectively solve the pain of patients with renal failure.