〔Abstract〕 Objective To analyze methicillin-resistantStaphylococcus aureus(MRSA) isolated from patients with skin and soft tissue infection(SSTI), and to analyze the homology of staphylococcal cassette chromosome mec(SCC mec), so as to provide laboratory basis for standardizing the rational use of antibiotics and nosocomial infection control. Methods 44 strains of MRSA isolated from patients with SSTI were tested for antimicrobial susceptibility by K-B method and Vitek 2 Compact instrument. MecA gene was detected by ordinary PCR, SCC mec genotyping was performed by multiplex PCR, and homology was analyzed by matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS). Results Mec A gene was detected in all 44 MRSA strains by common PCR detection. Three SCC mec genotypes of Ⅱ, Ⅲ and Ⅳa were identified by multiple PCR, including 5 strains of type Ⅱ(11.36%), 12 strains of type Ⅲ(27.27%), 8 strains of type Ⅳa(18.18%) and 19 strains of untyped type(43.18%). 44 MRSA strains were divided into two clusters by MALDI-TOF MS. Conclusion The main type of MRSA isolated from SSTI patients is SCCmec Ⅲ type. Through the combination of SCCmec genotyping and MALDI-TOF MS technology, it provides a basis for homology analysis among different strains and understands the molecular biology and epidemiological characteristics of MRSA in specific areas.