〔Abstract〕 Objective To investigate the clinical efficacy of injectable platelet-rich fibrin(i-PRF) combined with vacuum sealing drainage(VSD) technology in the treatment of chronic ulcers. Methods 80 patients with chronic ulcers were enrolled and randomly divided into control group and i-PRF group. In the control group, ulcers was blocked through the negative pressure drainage after wound debridement, and in the i-PRF group, ulcers were conducted multi-point dispersion injection of i-PRF on the wound surface and around the wound after wound debridement, followed by closed negative pressure drainage treatment. All patients repeated the above treatment in a 7-day cycle, and wounds was repaired after reaching the restore standard. The relevant parameters of the two groups were recorded, evaluated and compared on 7 th day and 14 th day after treatment, including bacterial culture, leukocyte, C-reactive protein, erythrocyte sedimentation rate, fresh granulation tissue growth and NRS score, preparation time for the second-stage repair surgery and the time for complete wound healing. Results The inflammation indexes and pain scores decreased in both groups on 7 th and 14 th day after treatment, while i-PRF group showed a more significant downward trend(P<0.05); i-PRF group had higher coverage of fresh granulation tissue and higher rate of bacterial conversion on 7 th and 14 th day after surgery, and the difference was statistically significant(P<0.05); i-PRF group had significantly lower preparation time for secondary repair surgery and complete wound healing time than VSD group(P<0.05). Conclusion Compared with the single application of VSD technology, i-PRF combined with VSD can play a more effective role in treating chronic wounds, controlling infection, reducing pain, accelerating wound healing and reducing hospitalization time.