〔Abstract〕 Cytomics FC500 flow cytometer and ImageStreamX Mark Ⅱ imaging flow cytometer were used to detect the percentage of mouse-derived RAW264.7 cells transfected with control group NC mimics and microRNA-22(miR-22) mimics to phagocytose mouse-derived glioma cells GL261, and the correlation and consistency of the two detection technologies were observed. The results showed that the ratio of RAW264.7 cells(NC mimics and miR-22 mimics) phagocytosing GL261 cells measured by two kinds of flow cytometry had a good correlation(R2>0.8). The Bland-Altman method analyzed the two detection techniques and found that the proportion of RAW264.7 cells(NC mimics and miR-22 mimics) phagocytosing GL261 cells was less biased, which were 4.289% and 8.073%, respectively. Compared with the control group NC mimics, the ability of RAW264.7 cells in miR-22 mimics group to swallow tumor cells increased(P<0.05), and imaging flow cytometry could also obtain individual cell pictures of the phagocytic process. Classical flow cytometry is easy to operate and fast while imaging flow cytometry is based on traditional flow cytometry, which realizes multi-parameter quantitative analysis of cell images and can obtain new statistical data of cell morphology.