〔Abstract〕 Objective To analyze the porcine wound exudatesin proteome and N-terminome at multiple time points after burn, and to determine the candidate biomarkers for evaluating the critical turning point of wound progression. Methods Eight Tibetan miniature pigs were randomly divided into 4 groups, 2 pigs in each group: 0 to 2 days(2 d),2 days to 4 days(4 d),4 days to 6 days(6 d),6 days to 8 days(8 d).4 groups of 5 cm deep second degree skin burn wounds were made by solid alcohol burning for 30 seconds, and the ethylene oxide sterilized foam dressing was cut into appropriate size and placed in the wound to collect the wound exudate.Using a TAILS-iTRAQ,we had established a time-resolved proteome and N-terminome resource from wound exudates in a clinically relevant pig burn wound model that we exploited as a robust template to interpret a heterogeneous dataset from patients, and evaluated the prospective biomarker candidates for assessment of critical turning points in wound progression. Results 1 060 quantifiable proteins and 1667 neo-N-termini from porcine wound exudates were collected.By one-way analysis of variance, we identified 416 proteins and 371 neo–N-termini with significant changes in abundance over time and a subset of 188 neo–N-termini that significantly changed in relation to the abundance of the whole protein(cleavages). IPA showed a prevalence of pathways associated with inflammation(reactive oxygen species/nitric oxide synthase in macrophages, acute phase signaling, complement system) and immediate consequences of injury(coagulation system) in clusters comprising proteins with high abundances in the middle(d4,d6)(clusters 2 and 3) and of pathways associated with cell proliferation and migration(remodeling of epithelial adherens junctions) in clusters including proteins and related neo–N-termini with high abundances at the end(d8) of the monitored healing phase(clusters 4 and 5).The correlation of time-resolved protein abundance profiles derived from the pig wound model and from patient data showed a very high correlation(0.84–0.98) of changes in abundance of proteins associated with dynamics of epithelial adherens junctions.ZYX,IQGA1 and HtrA1 could be used as markers of wound healing process in wound exudate of patients treated with negative pressure suction. Conclusion By using iTRAQ-TAILS to analyze the wound exudate from a pig burn model, many known and novel proteolytic processing eventsin vivoare identified, reflecting the key domain of protease activity in this highly complex tissue reaction.