〔Abstract〕 Objective To develop a nano particle delivery system which combined deacetylated poly(N-acetylglucosamine)(DEAC-PNAG) polymer with miR-150 by self-assembly and the corresponding vascular protection mechanism were discussed. Methods Nano polymer was obtained by self-assembly, characterized by transmission electron microscopy and DLS.The gel displacement method was used to evaluate the encapsulation efficiency.Cell uptake was detected by fluorescence microscopy. RT-PCR was used to detect the expression of miR-150 in transfected cells.Western blot was used to detect the effect of nano-polymer on the expression of Ang-Ⅱ and DLK-1 in HUVECs. Results DEAC-PNAG polymer with nano size and positive surface potential could protect miRNA from RNase A degradation.In addition, DEAC-PNAG@miRNA could promote the cell uptake of miRNA and play a role in vascular protection by inhibiting the expression of Ang-Ⅱ and DLK-1 in HUVECs. Conclusion DEAC-PNAG is an effective miRNAs delivery platform, which may have positive significance for sepsis treatment.