〔Abstract〕 Objective To investigate the effect of long non-coding RNA(LncRNA) H19 on the apoptosis of rat pancreatic acinar cells AR42 J induced by caerulein. Methods An AP modelin vitrowas established by stimulating with caerulein. The mRNA and protein expression levels of Caspase 3 and Caspase 9 were detected by qRT-PCR and Western blot after H19 silencing and overexpression. The apoptosis rate of AR42 J cells in each group was detected by Annexin V-FITC/PI assay. Results After H19 silencing, the mRNA and protein levels of Caspase 3 and Caspase 9 significantly decreased, and the apoptosis rate was also relatively reduced(P<0.05). After H19 overexpression, the mRNA and protein levels of Caspase 3 and Caspase 9 significantly increased, and the apoptosis rate also significantly increased(P<0.05). Conclusion The silencing of LncRNA H19 can significantly reduce the apoptosis of cerulean-induced pancreatic acinar cells, suggesting that it may be a potential effective target for the treatment of acute pancreatitis.