〔Abstract〕 Objective To investigate the effect of Mitoquinone(MitoQ) on the migration and apoptosis of human lung adenocarcinoma A549 cells through NIX-mediated mitochondrial autophagy. Methods A549 cells were exposed to various concentrations of MitoQ for 12 hours, the Cell Counting Kit-8(CCK-8) assay was used to detect cell viability. Cell migration was measured with scratch assay. Western blot analysis was used to detect the expression levels of the mitochondrial autophagy and apoptosis related proteins NIX, p62 and Bax. Results Compared with that in the control group, MitoQ groups showed significant inhibition of proliferation and migrate. Accompanied by the occurrence of apoptosis, the expression of autophagy-promoting related proteins(NIX) was up-regulated, the expression of autophagy-inhibiting related proteins(p62) was down-regulated, the expression of apoptosis-promoting related proteins(Bax) was up-regulated, and the difference increased with elevating MitoQ concentration(P<0.05); compared to the control group, MitoQ groups exhibited a decreased capacity for cell migrate and proliferation, and an increased levels of mitochondrial autophagy and apoptosis. Conclusion MitoQ can inhibit A549 cells migration and proliferation, and promote A549 cells apoptosis. The mechanism may be related to the MitoQ regulating key molecules on the mitochondrial autophagy pathway and inducing autophagy in A549 cells, and the key molecule of mitochondrial autophagy may be the NIX protein.