〔Abstract〕 Objective To investigate the effect of knocking out hsa_circRNA_102958 on the proliferation, oxidative stress and mitochondrial function of pancreatic cancer PANC-1 cells. Methods Pancreatic cancer PANC-1 cells were transfected with shRNA-NC and three different circ_102958 shRNA series. RT-PCR was used to detect the expression of circRNA_102958. The best interference effect was selected for circ_102958 shRNA1 for follow-up experiments. CCK8 was used to detect cell proliferation multiples; Hoechst staining was used to observe cell morphology changes; total superoxide dismutase(SOD) activity detection kit, malondialdehyde(MDA) detection kit and DCFH-DA kit were used to measure SOD, MDA, and active oxygen(ROS)content respectively; flow cytometry was used to detect JC-1 red/green fluorescence ratio; Western blot was used to detect Bax/Bcl-2, cleaved cas9/cas9, cleaved cas3/cas3, c-Myc protein expression. Results Compared with the Control group, interfering with the expression of circRNA_102958 inhibited the proliferation of pancreatic cancer PANC-1 cells(P<0.05), the cell morphology tended to be disordered, apoptotic cells increased, and the contents of MDA and ROS were up-regulated(P<0.05). SOD activity decreased(P<0.05), the ratio of red/green fluorescence of JC-1 was down-regulated(P<0.05), and the protein expression of Bax/Bcl-2, cleaved cas9/cas9 and cleaved cas3/cas3 increased(P<0.05). Conclusion Interfering with the expression of circRNA_102958 can inhibit the proliferation and mitochondrial function of pancreatic cancer PANC-1 cells, and promote cell apoptosis and oxidative stress.