〔Abstract〕 To evaluate the diagnostic and differential ability of RV1737 peptide antigens in tuberculosis and the peptide immunoreactivity which was based on the cellular immune simulated interferon gamma(IFN-γ) release test(IGRA) method. After the action of peptides A, B and C, the IFN-γ level of the latent tuberculosis(LTBI) group was significantly higher than that of the comparative Tuberculosis(TB) group(P<0.05). The sensitivity of the combination group A+B+C was significantly higher than that of peptides A, B, C and fusion D(P<0.05). The antibody of tuberculosis IgG was detected by indirect ELISA based on polypeptide. Better reactivity in TB group serum, the absorbance of IgG antibody of polypeptide A in the TB group was significantly greater than that of polypeptides B and C(P<0.05). The area of polypeptide A was the largest when ROC was drawn between groups, and when combined with A+B+C polypeptide, the curve area increased and the difference was statistically significant(P<0.05). RV1737 polypeptides may be candidate antigens for the diagnosis of latent tuberculosis, ELISA may be established to detect peripheral blood TB IgG antibody by polypeptide antigen. In particular, polypeptide A may be the new antigen for screening serological experimental tuberculosis.