〔Abstract〕 Objective To study the effect of eupatilin on miR-188-5 p overexpression and its effect on the proliferation, invasion and apoptosis of retinoblastoma. Methods Y79 cells were selected for research in retinoblastoma lines. Different concentrations of eupatilin(1, 2.5, 5, 10, 20, 40, 100, 200, 300 μmol/L) were used to treat Y79 cells and MTT assay was used to detect cell viability. Y79 cells were cultured with 0, 10, 20, 40 μmol/L eupatilin, and the effects of eupatilin on proliferation, apoptosis and invasion of Y79 cells were tested. Control group, mimic-NC transfection group, miR-188-5 p mimic transfection group were established, then the cells were treated with concentration of 40 μmol/L eupatilin each group to detect the effect of eupatilin on the expression of miR-188-5 p, the proliferation, apoptosis and invasion of Y79 cells. RT-qPCR was used to detect the expression of miR-188-5 p, colony formation test, flow cytometry and Transwell test were used to detect cell proliferation, apoptosis and invasion.Western blot was used to detect the protein expression of E-cadherin, N-cadherin, Bax and Bcl-2. Results MiR-188-5 p overexpression promoted the Y79 cells proliferation, reduced cell apoptosis and increased cell invasion. Eupatilin could inhibit miR-188-5 p overexpression, thereby inhibiting retinoblastoma Y79 cell proliferation, inducing apoptosis. Conclusion Eupatilin inhibits the cell invasion and induces apoptosis of retinoblastoma by inhibiting the expression of miR-188-5 p.