〔Abstract〕 Objective To observe its effect on the proliferation, invasion and apoptosis of human glioma cells by the overexpression recombinant plasmid targeting to long non-coding maternally expressed gene 3(LncRNA MEG3) gene transfects the human glioma cells in vitro.Methods The expression of LncRNA MEG3 mRNA in normal brain tissue cells(N) and glioma cell line U251 cells was detected by qRT-PCR. Eukaryotic overexpression vector ofpCI-MEG3 was constructed to transfect the human glioma cell line U251 cells as pCI-MEG3 overexpression plasmid group(pCI-MEG3 group). Those transfected with empty pCI plasmid were divided into empty vector pCI group(pCI group) and those without any transfection were divided into Control group. The expression of LncRNA MEG3 gene was analyzed by qRT-PCR. The expression of MDM2 and P53 of LncRNA MEG3-related protein was confirmed by Western blot. The proliferation ability of glioma cells was analyzed by techniques of MTT and cell cloning. Transwell was used to analyze the invasion ability of glioma cells,and flow cytometry was used to analyze the apoptosis rate of glioma cells. Results Compared with normal brain tissue cells,the expression level of LncRNA MEG3 mRNA decreased in glioma cells. The results of vitro experiments showed that both the mRNA of LncRNA MEG3 and protein expression levels of p53 increased,and the expression levels of MDM2 protein were down-regulated( P<0. 05) in p CI-MEG3 group when compared with the control group and the p CI group( P<0. 05). Cell proliferation and cell invasion were inhibited( P<0. 01). The apoptosis rate increased( P<0. 01). Conclusion The expression of LncRNA MEG3 in human glioma cells is lower than that in the normal human brain tissue cells. Overexpression of LncRNA MEG3 in human glioma cells can increase the expression of LncRNA MEG3 thereby inhibiting the proliferation and invasion of human glioma cells and increasing the apoptosis rate.