〔Abstract〕 Objective To study the effect of miR101 on glucose absorption in placental trophoblast by regulating EZH2 protein, and the possible mechanism of miRNA101 in gestational diabetes mellitus(GDM). Methods The toatal of 30 pregnant women with GDM and 30 normal pregnant women were selected as the observation group and the control group respectively. The expression of miR101 and EZH2 mRNA in placental tissue was detected by qRT-PCR, and the expression of EZH2 protein in placental tissue was detected by Western blot. Human chorionic trophoblast cells were transfected with mir101 mimic, mir101 mimic control sequence, mir101 inhibitor and mir101 inhibitor control sequence as M group, M-NC group, I group and I-NC group respectively. Blank control group B was set up with 6 parallel multiple pores in each group. qRT-PCR was used to detect miR101 and EZH2 mRNA expression. Western blot was used to detect EZH2 protein expression, and glucose detection kit was used to detect glucose uptake. Results The expression level of miR101 in the observation group was significantly higher than that in the control group(t=5.433,P<0.05), and the expression level of EZH2 in the observation group was lower than that in the control group(t=2.178,P<0.05). After cell transfer:(1) The expression level of miR101 in group M was significantly higher than that in group B and M-NC(t=2.133,2.167,P<0.05). The expression level of miR101 in group M was significantly lower than that in group B and I-NC(t=2.918, 2.331,P<0.05).(2) The expression of EZH2 in group M was significantly lower than that in group B and M-NC(t=2.018, 2.257,P<0.05). The expression level of EZH2 in group M was significantly lower than that in group B and I-NC(t=3.271, 3.167,P<0.05).(3) Glucose consumption in group M was lower than that in group B and M-NC(t=3.106, 2.115,P<0.05). Glucose consumption in group M was higher than that in group B and I-NC(t=2.002, 2.158,P<0.05). Conclusion The expression of miR101 in patients with GDM placental trophoblast abnormally increase. MiR101 could regulate the expression of EZH2 and decrease the absorption of glucose by placental trophoblasts, which was involved in the pathological mechanism of GDM.