〔Abstract〕 Objective To study the polarization of macrophages induced bytoxoplasma gondiiWh3 strain. Methods Macrophages THP-1 were infected withtoxoplasma gondiiWh3 strain, RH strain and ME49 strain. After 6 hours and 24 hours of infection, the supernatant and macrophages were collected. The concentrations of IL-6, IL-10, TNF-α and TGF-β of the supernatant were detected by ELISA. In macrophages, the mRNA transcription levels of M1/M2 related genes iNOS, arginase-1 and TNF-α, IL-12, TGF-β, IL-10 were detected by qRT-PCR, and the expression levels of iNOS and arginase-1 were analyzed by Western blot. Results The results of ELISA showed that the contents of IL-6 and TNF-α were the highest after infection by ME49 strain 6 hours and 24 hours(P<0.01), and the highest of IL-10 and TGF-β were detected after infected with RH strain(P<0.01). However, the content of cytokines in supernatant infection by Wh3 strain was between RH strain and ME49 strain. Some inflammatory factors and cytokines mRNA in macrophages were detected after 6 hours and 24 hours of infection, and iNOS, TNF-α and IL-12 significantly increased after infected withtoxoplasma gondiiME49 strain(P<0.05), arginase-1, IL-10 and TGF-β significantly increased after RH strain infection. It was also found that the levels of mRNA in macrophages infected with Wh3 strain was between RH strain and ME49 strain. After 24 hours of infection, the expression of iNOS protein increased with wh3 and me49 strains oftoxoplasma gondii(P<0.000 1), while the expression of arginase-1 increased with RH strain(P<0.001). Conclusion The polarization direction of macrophages was between M1 and M2 after infection withtoxoplasma gondiiWh3 strain, and it was closer to M2.