〔Abstract〕 Objective To investigate the effect of miR-455-3 p on the growth and movement of ovarian epithelial cancer cells by targeting HDAC2. Methods Luciferase assay was performed to verify the targeting relationship between miR-455-3 p and HDAC2. HDAC2 pcDNA vector was constructed to overexpress HDAC2 and the cells were transfected into mimic. The cells were randomly divided into four groups: Control group, mimic group, HDAC2 group and mimic+HDAC2 group for subsequent experiments. BRDU staining was used to detect cell proliferation. Cell apoptosis was detected by flow cytometry. The mRNA levels of Ki67, PCNA and c-Myc were detected by RT-PCR. Transwell detected cell invasion. Cell migration was detected by scratch test. Western blot detected vascular endothelial growth factor(VEGF), Vimentin and Fibronectin protein expression levels. Results The results showed that miR-455-3 p had directly targets with HDAC2, compared with HDAC2 group, the number of BRDU positive cells in mimic+HDAC2 group significantly reduced(P<0.05), cell apoptosis rate increased significantly(P<0.05), Ki67, PCNA, c-Myc mRNA level decreased significantly(P<0.05), invasive cells significantly reduced(P<0.05), nick healing rate significantly decreased(P<0.05), VEGF, Vimentin and Fibronectin protein levels significantly decreased(P<0.05). Conclusion miR-455-3 p can induce HO-8910 apoptosis of ovarian cancer cells by targeting HDAC2, inhibit cell proliferation, invasion and migration, and reduce movement ability.