〔Abstract〕 Objective To evaluate the diagnostic value of acoustic structure quantification(ASQ) technique in chronic hepatitis b cirrhosis and non-alcoholic fatty liver disease. Methods Thirty healthy volunteers, forty patients with chronic hepatitis b cirrhosis and thirty patients with non-alcoholic fatty liver disease were examined by ASQ. The ultrasonic two-dimensional and ASQ images were collected and analyzed. The relevant parameters(redmode, redave, redSD, bluemode, blueave, blueSD and FD ratio)were statistically analyzed. Receiver-operating characteristic(ROC)curves were drew for each parameter. Results The difference of red line parameters was statistically significant between each two groups(P<0.05). BlueSD showed statistically significant differences among all groups except the normal control group and the chronic hepatitis b cirrhosis group(P<0.05), while the difference of bluemode and blueave was statistically significant between each two groups(P<0.05). There were statistically significant differences in FD-ratio between all groups except the normal control group and the non-alcoholic fatty liver group(P<0.05). In chronic hepatitis b cirrhosis group,the ROC evaluation showed that the area under the cure(AUC)of redmode, redave, bluemode and FD ratio(0.940, 0.990, 0.745, 0.920)were greater than 0.7, with higher diagnostic value. The corresponding critical values were 108.32,111.19,15.50,126.84,145.60,26.82 and 0.16 respectively. In the non-alcoholic fatty liver group, the ROC evaluation showed that the AUC of each parameter(0.964,0.951,0.864,0.894,0.852,0.749,0.898)was greater than 0.7, indicating higher diagnostic value. The corresponding critical values were 100.64,104.31,13.95,119.28,126.99,20.08 and 0.09 respectively. Conclusion As a new non-invasive and objective examination method, ASQ technology can not only quantitatively diagnose these two diseases, but also has high value in the diagnosis of non-alcoholic fatty liver disease.