〔Abstract〕 Objective To explore the feasibility of the bilayer chitosan barrier membrane loaded with tannic acid(CS@TA) for guided bone regeneration by exploring the reactive oxygen species(ROS) scavenging ability, biocompatibility, and antibacterial properties. Methods The single-layer chitosan(CS) film was prepared by self-evaporation, and the double-layer CS film was prepared by directional freezing and freeze-drying, and its microstructure was observed by scanning electron microscope. The prepared CS bilayer membrane was grafted with tannic acid(TA) in different proportions, and the interaction between TA and CS bilayer membrane was analyzed by Fourier infrared spectrometer(FITR). The ROS scavenging ability was tested by 1,1-diphenyl-2-picrylhydrazyl(DPPH), and the double-layer membrane of loading TA scavenging efficiency of more than 90% was selected to continue the follow-up experiment. CCK-8 assay and lived dead staining were used to evaluate the survival rate of cell in each groups. MC3T3-E1 cells was adhesion the CS@TA barrier film for studying by SEM. Colony counting was performed to test its antibacterial performance againstEscherichia coli(E.coli) andStaphylococcus aureus(S.aureus). Results One side was the smooth, dense while other side was rough, loose and porous, with a longitudinal ordered porous structure in cross-section of the double-layer membrane of CS@TA. With the addition of TA, the ROS scavenging ability of the double-layer membrane first increased rapidly and then stabilized slowly. The results of CCK-8 and lived and dead cells staining showed that excessive TA addition significantly affected the biocompatibility of the double-layer membrane. The counting results of bacterial dilution coating showed that compared with the double-layer membrane without TA loading, the double-layer membrane had certain antibacterial ability againstE.coliandS.aureuswhen the appropriate amount of TA was added. Conclusion Thus the double-layer with appropriate TA loading has strong ROS scavenging ability, good biological performance, and certain antibacterial ability forE.coliandS.aureus.