〔Abstract〕 Objective To investigate the mechanism of tanshinone ⅡA(TSA) regulating PTEN on the autophagy pathway PI3 K/AKT/mTOR after cerebral ischemia in rats. Methods Rat model of cerebral ischemia(MCAO) was established and treated with TSA. The experiment was divided into three groups:normal control group, MCAO+ saline(NS) group and MCAO+TSA group. Immunohistochemistry and Western blot were used to detect the protein expression. Results Compared with the control group, the expression of PTEN protein in the MCAO+TSA group decreased, the levels of PI3 K, AKT and mTOR increased, and the difference was statistically significant(P<0.05). Compared with the two groups in the surgery group, the PTEN protein in the TSA group decreased significantly compared with the MCAO group, while the expression levels of PI3 K, AKT and mTOR in the TSA group increased slightly compared with the MCAO group, and the difference was statistically significant(P<0.05). Conclusion By enhancing the expression of PTEN in rats with cerebral infarction, TSA can reduce the expression level of PI3 K/AKT/mTOR in autophagy pathway, reduce the inflammation of cerebral infarction cells, so as to protect the brain tissue of rats.