〔Abstract〕 Abstrsct Objective To determine the metabolite of intestinal bacteria,D-lactic acid( D-LA),the product of intestinal mucosa,diamine oxidase( DAO) and markers of inflammation in male patients with alcoholic fatty liver disease( AFLD) and male patients with nonalcoholic fatty liver disease( NAFLD),and to investigate the changes of intestinal mechanical barrier function in male patients with fatty liver disease( FLD). Methods A total of 40 male patients with alcoholic fatty liver disease( AFLD group),91 male patients with nonalcoholic fatty liver disease( NAFLD group),and 82 normal people( normal control group) were selected as the object of study. Questionnaires were sent to them,followed by physical examination,liver ultrasound examination and blood eamination.The physical examination report was collected. A self-designed fatty liver questionnaire was used to investigate the general condition of individuals. Venous blood was sampled to test biochemical indexes,intestinal permeability and inflammatory makers of body. Results The body mass index( BMI),alanine aminotransferase( ALT),aspartate aminotransferase( AST),triglycerides( TG),very low-density lipoprotein cholesterol( VLDL-C),and diamine oxidase( DAO) in NAFLD group and AFLD group were all higher than those in the control group. The high-density lipoprotein cholesterol( HDL-C) in NAFLD group and AFLD group was lower than that in the control group. The concentration of serum D-lactic acid( D-LA) in NAFLD group was higher than that in the control group. All these differences above were statistically significant( P<0. 05). Inflammatory makers suggested that the serum level of tumor necrosis factor-α( TNF-α),interleukin-6(IL-6) and C-reactive protein(CRP) in NAFLD group and AFLD group was higher than that in the control group,and the concentration of TNF-α and CRP in AFLD group was higher than that in NAFLD group(P<0. 05). The correlation analysis showed that the DAO in serum was positively correlated with ALT,AST,TC,TG,and VLDL-C(rs>0,P<0.05),the DAO and D-LA in serum were negatively correlated with HDL-C(rs<0,P<0. 05),and the TNF-α,IL-6 and interleukin-1β(IL-1β) in serum were positively correlated with DAO( rs>0,P<0.05). Conclusion The level of DAO and D-LA in serum of NAFLD patients significantly increased,while the DAO in serum also increased in AFLD patients,suggesting that intestinal mechanical barrier function in patients with fatty liver disease was impaired compared with normal population. The increased serum level of TNF-α,IL-6 and CRP in patients with NAFLD and AFLD,and the level of TNF-α and CRP in AFLD patients was higher than that in NAFLD patients,suggesting that the inflammatory level in fatty liver disease was higher,and the inflammatory level in AFLD patients might be higher.