〔Abstract〕 Objective To develop a high modulus and high strength biodegradable silk fibroin GBR membrane to address the issue of maintaining the space for bone regeneration in the repair of osseous defects. Methods After purifying silk fibroin protein, membrane materials were prepared using evaporation-hot pressing method. The physical and chemical properties and biological performance of the membranes were evaluated using stretching tests,in vitrosimulations, and cell co-culturing methods. Results A silk fibroin GBR membrane was successfully fabricated, resulting in a simulated degradation rate of 35.3% after 12 hin vitro. The wet-state elastic modulus reached 45 MPa, while the tensile strength reached 8.39 MPa. Furthermore, the cell survival rate was nearly 100% after 7 days. Conclusion The biodegradable GBR membrane produced in this study possesses high modulus and strength, as well as excellent biocompatibility, offering a promise as a foundation for addressing the bone defect repair and bone space maintenance.