〔Abstract〕 Objective To investigate the relationship between the methylation of growth inhibitory factor 4(ING4) gene and cervical carcinogenesis and its clinical significance. Methods Tissue specimens of 80 patients with cervical cancer diagnosed were collected, and 50 cervical tissue specimens from patients with benign uterine lesions undergoing total hysterectomy during the same period were used as the control group. Methylation-specific PCR(MSP) technique was used to detect the methylation status of the ING4 gene promoter in cervical cancer tissues and cervical tissues of the control group, and the mRNA expression level of ING4 gene was detected by real-time quantitative reverse transcriptase-polymerase chain reaction(qRT-PCR). The expression level of ING4 mRNA was analyzed, and the relationship between the methylation status of ING4 gene promoter and the clinical case characteristics of cervical cancer was analyzed. Results The methylation rate of ING4 gene in tissue of cervical cancer(66/80, 82.5%) was significantly higher than that of control group(6/50, 12%)(P<0.01). The mRNA expression of ING4 in cervical cancer tissue(0.41±0.07) was lower than that in control group(0.87±0.16). In addition, the mRNA level in cervical cancer tissues methylated by the ING4 promoter region(0.37±0.06, n = 66) was lower than that in tissues of cervical cancer patients with unmethylated ING4 promoter region(0.46±0.11, n=14). There was no statistically significant difference between the methylation of ING4 gene promoter region and age, lymph node status, pathological classification and clinical stage of cervical cancer in cervical cancer tissues(P>0.05). Conclusion Hypermethylation of the ING4 gene promoter region may be associated with the occurrence and development of cervical cancer.