〔Abstract〕 Objective To evaluate the effects of mouse bone marrow mast cell derived exosomes on the proliferation, migration and invasion of hepatocellular carcinoma Hepa1-6 cells. Methods Mouse bone marrow mast cells were cultured by using IL-3 and SCF cytokinesin vitro. The cells were identified by toluidine blue staining, immunofluorescence and flow cytometry. Exosomes were isolated by ultracentrifugation from supernatants, and the morphology, diameter and marker protein of exosomes were detected by transmission electron microscopy, particle size analysis and Western blot. Hepa1-6 cells were treated with different concentrations of exosomes(5, 10, 20 μg/ml). CCK-8 test, Edu test, scratch test and transwell test were used to detect the proliferation, migration and invasion of hepatocellular carcinoma cells. Results The exosomes of bone marrow mast cells were round or oval cup shape, with a particle size of 30～150 nm, and expressed exosomal marker proteins CD63, Alix, and TSG101.The results of CCK-8 and Edu showed that exosomes derived from mast cells promoted the proliferation of Hepa1-6 cells, and the effect is concentration-dependent. Cell scarification results showed that exosomes derived from mast cells promoted the migration of Hepa1-6 cells. Transwell results showed that exosomes derived from mast cells enhanced the invasion ability of Hepa1-6 cells. Conclusion Bone marrow mast cell derived exosomes can promote the proliferation, migration and invasion of hepatocellular carcinoma Hepa1-6 cells.