〔Abstract〕 Objective To investigate the expression of fusion protein HSPE1-MOB4 in heat shock protein family member 1(HSPE1) and murine human-like alpha-chemokine analog1(MOB4) in different cells. Methods Reverse transcription polymerase-chain reaction(RT-PCR) was used to detect the expression of fusion protein HSPE1-MOB4 in different cells. Sanger gene sequencing was used to explore different subtypes of fusion protein. Protein position in cells was detected by immunofluorescence technique and laser confocal microscopy.The expression of fusion protein HSPE1-MOB4 in normal cells and tumor cells was detected by immunoblotting. Results The fusion protein HSPE1-MOB4 was expressed in normal cells and tumor cells. The results of gene sequencing showed that the fusion protein HSPE1-MOB4 had two different subtypes, and the expression positions of the two subtypes were the same. Compared with normal cells, the amount of fusion protein expressed in tumor cells was higher. Conclusion The fusion protein HSPE1-MOB4 has two subtypes and the reported one is highly expressed in tumor cells. This may provide new ideas for finding new tumor biomarkers.