〔Abstract〕 A simple and efficient method of human ovarian serous adenocarcinoma cells(HOSACs), culture was established through improving methods for culturing primary cells of the common human ovarian serous adenocarcinoma. 25 tissue specimens of serous ovarian carcinoma confirmed by clinicopathology were collected, and each specimen was randomly divided into two groups: the modified group and the traditional group. The modified group was extracted cells with modified enzyme digestion method and tissue block adherence method(trypsin+hyaluronidase digestion for 2～3 min+tissue block and cell suspension plating). Traditional group was extracted with tissue block adherence method(the tissue block is shredded directly and then plating). The cell purity and cell viability obtained by the two methods were compared by cell immunofluorescence and CCK-8. The growth characteristics of primary cells of the two groups were observed under phase contrast microscope at 48 h and 72 h. The results of immunofluorescence and CCK-8 showed that the number of cells, cell viability and cell purity obtained in the modified group were higher than those in the traditional group(P<0.05).