〔Abstract〕 Objective To investigate the effect of protein arginine methyltransferase(PRMT1) on gene expression of PI3 K-AKT pathway in gastric cancer. Methods The differentially expressed genes between 2 cases of gastric cancer and 2 cases of adjacent tissues were screened by transcriptome sequencing. The key genes affecting the pathway of gastric cancer were enriched and analyzed by kyoto gene encyclopedia of genome(KEGG) and verified by immunohistochemistry. Results 758 differentially expressed genes with length greater than 200 bp were obtained by high throughput sequencing. KEGG enrichment analysis revealed 25 significant enrichment metabolic pathways, of which PI3 K-AKT metabolic pathway was significantly abnormally activated containing 15 genes significantly up-regulated. cBioportal database correlation analysis showed that PRMT1 was negatively correlated with the expression of key genes(PPP2 R3 A, THBS4) in PI3 K-AKT signaling pathway. Immunohistochemical results further showed that there was a high expression of p-AKT(473 Ser) in gastric cancer tissues with low PRMT1 expression, moreover, there was a negative correlation between PRMT1 and p-AKT(473 Ser), indicating that PRMT1 was negatively correlated with PI3 K-AKT signal activity. Conclusion PRMT1 may affect the activity of PI3 K-AKT signaling pathway in gastric cancer through PPP2 R3 A and THBS4, which may provide clues for finding diagnostic markers and therapeutic targets of gastric cancer.