〔Abstract〕 Objective To investigate the expression of interleukin-11(IL-11) in glioma and its effect on glioma proliferation, migration and invasion. Methods qRT-PCR was performed to determine the expression levels of IL-11 in clinical samples, glioma cell lines and SF126 cells transfected with IL-11 knockdown plasmid. SF126 glioma cells were tansfected with IL-11 knockdown plasmid(shIL-11) or treated with recombinant human IL-11(rhIL-11), and then the colony formation assay was performed to detect the proliferation of glioma cells, and Transwell assay was used to detecte cell migration and invasion. Results The expression of IL-11 mRNA in glioma tissue was higher than that in normal brain tissue. Colony formation assay and transwell assay indicated that knockdown of IL-11 inhibited proliferation, migration and invasion of glioma cells. In contrast, SF126 cells treated with rhIL-11 significantly promoted proliferative, invasive and migratory capacity. Conclusion IL-11 is upregulated in glioma cells and promotes proliferation, migration and invasion ability.