〔Abstract〕 Objective To investigate the effect and mechanism of microRNA miR-206 on the growth and motility of ovarian cancer cell CAOV3.Methods The targeted gene of miR-206 was predicted by bioinformatics and its targeted-relationship were confirmed by the luciferase reporter assay. Cells were divided into Control, miR-206 mimic,pc-MAPK1 and miR-206 mimic + pc-MAPK1 group,the expression of miR-206 was measured by qRT-PCR,cell growth was measured by Brdu staining,cell invasion was measured by Transwell,the expression of Mitogen-activated protein kinase 1( MAPK1),epithelial cadherin( E-cadherin),vimentin( vimentin),c AMP response element binding protein( CREB),phosphorylated CREB( P-CREB),Ets-like Transcription factor-1( Elk-1),P-Elk-1,proto-oncogene( c-Myc),P-c-Myc and vascular endothelial growth factor( VEGF) were measured by Western blot.Subcutaneous xenograft tumors of nude mice with miR-206 transfected or non-transfected CAOV3 cells were established. The volume of the transplanted tumors was measured. The expression levels of miR-206 and MAPK1 were detected by qRT-PCR. The expression levels of Elk-1,P-Elk-1,c-Myc and Pc-Myc expression was detected by Western blot. Ki67 and vimentin expression were detected by immunohistochemistry. Results miR-206 targeted the inhibition of MAPK1 expression. Compared with the control group,the percentage of Brd U positive cells and the number of invasive cells in the miR-206 mimic group reduced( t = 4. 27,P = 0. 002; t = 3. 43,P = 0. 008),the expression of E-cadherin increased( t = 4. 13,P = 0. 003),P-CREB/CREB,P-Elk-1/Elk-1,Pc-Myc/c-Myc ratios and the expression of vimentin were down-regulated( t = 3. 27,P = 0. 010; t = 3. 26,P = 0. 010; t = 3. 28,P = 0. 010,t =3. 51,P = 0. 007). miR-206 mimic reduced the volume of subcutaneously transplanted tumors of CAOV3 cells in nude mice( t = 6. 51,P<0. 001),increased the expression of miR-206 in the tissues of transplanted tumors( t =4. 65,P = 0. 001),reduced MAPK1 mRNA levels,VEGF expression,P-Elk-1/Elk-1 and Pc-Myc/c-Myc ratio( t =3. 86,P = 0. 004; t = 3. 37,P = 0. 009; t = 3. 34,P = 0. 009; t = 3. 40,P = 0. 008),and reduced the percentage of Ki67 and Vimentin positive cells( t = 4. 45,P = 0. 002; t = 4. 22,P = 0. 002). Conclusion miR-206 over expression can inhibit ovarian carcinoma cell CAOV3 growth and mobility via obstructed activation of MAPK signal pathway.