〔Abstract〕 To explore the correlation between the serum level of 25 hydroxy vitamin D3[25-(OH)D3] with ovarian responsibility and the pregnancy outcomes of frozen-thawed embryo transfer(FET). 157 patients with tubal infertile factors who received detection of vitamin D before in vitro fertilization and embryos transfer(IVF) were enrolled this study, and assigned into two groups according to the serum level of 25-(OH)D3:25-(OH)VD3<20 μg/L group and 25-(OH)D3≥20 μg/L group. The outcomes of ovarian stimulation and FET were compared between two groups. No statistically significant difference was found in patients' retrieved oocytes, MⅡ, fertilization rate, total dosage duration of gonadotropin(Gn), the total day of Gn injection, high-quality embryonic rate and E2in HCG day(P>0.05). Whereas, clinical pregnancy rate in the 25-(OH)D3sufficiency group(64.3%)was significantly higher than that in 25-(OH)D3deficiency group(45.5%). Although serum 25-(OH)D3has less effect on ovarian responsibility, serum 25-(OH)D3deficiency may have adverse effect on the pregnancy rate of FET.