〔Abstract〕 Objective To investigate the changes of vascular endothelial growth factor(VEGF) and epithelial kinase A2(EphA2) in human umbilical vein endothelial cells(HUVECs) apoptosis induced by brucine. Methods HUVECs cultured in conventional culture,which were divided into blank control group, treatment group with low brucine(10 μmol/L), medium(20 μmol/L) and high(40 μmol/L). HUVECs cell activity was detected by CCK-8 assay, migration of HUVECs was detected by scratch assay, and apoptosis of HUVECs cells was detected by immunofluorescence of Hoechst 33258 and flow cytometry with Annexin V/PI double staining. The expression of VEGF and EphA2 in HUVECs cells was detected by Western blot. Results Brucine could inhibit HUVECs proliferation in a concentration-dependent manner. Scratch test showed that brucine could inhibit HUVECs migration. Immunofluorescence and flow cytometry showed that brucine promoted HUVECs apoptosis in a concentration-dependent manner, and Western blot results showed that brucine down-regulated protein expressions of VEGF and EphA2 in HUVECs in a concentration-dependent manner. Conclusion Brucine can inhibit the proliferation and migration of HUVECs, and induce apoptosis, which may be related to the down-regulated expression of VEGF and EphA2.