〔Abstract〕 Objective To observe the expression of autophagy-related microRNAs in renal tubular epithelial exosomes under hypoxia. Methods Tubular epithelial cells were cultured in vitro and treated with normoxia and hypoxia respectively. ExoQuick-TC was used to extract the exosomes. The characteristics of the exosomes were quantitatively identified by transmission electron microscopy and protein concentration. RT-PCR was used to detect autophagy-related microRNA30 b, microRNA30 c, microRNA214 and miRNA-183,miRNA-200 a expression in the exosomes of tubular epithelial cells. Results Under hypoxic condition, the secretion of renal tubular cells in vitro did not change significantly, and the secretion of exosome/cell protein content significantly increased after 48 hours of hypoxia than that in normoxia group. With the prolongation of hypoxia time, the expression of autophagy-related exosome gradually increased. The levels of autophagy-related miRNA-30 b, miRNA-30 c, miRNA-214, miRNA-183 and miRNA-200 a in the hypoxia group were higher than those in the normoxic group, and gradually increased with the prolongation of hypoxia. Conclusion Hypoxia can alter the biological function of renal tubular cell secretory exosome, which is manifested by up-regulation of autophagy-related microRNA expression. This may be one of the reasons for the important organ autophagy in patients with chronic kidney disease.