〔Abstract〕 Objective To construct a lentiviral vector of long non-coding RNA(lncRNA) BC002811 and investigate the effect of stable BC002811 up-regulation on proliferation of gastric cancer HGC-27 cells. Methods BC002811 gene sequence was amplified by PCR, and was ligated with the pLVX-EGFP-IRES-neo vector. After being identified by double enzyme digestion and sequencing analysis, the recombinant vector was co-transfected into HEK293 T cell line with packaging plasmids to produce the lentiviral particles. Lentivirus was used to infect HGC-27 cells, and the cells with stably expressing BC002811 after selection with limiting dilution analysis were amplified and cultured. The expression level of BC002811 was detected by qPCR, and the cell proliferation capacity was determined by MTS. Results The BC002811 recombinant lentivirus plasmid was successfully constructed, which was confirmed by double enzyme digestion and DNA sequencing. The recombinant lentiviral vector was packaged in HEK293 T cells and the titer of concentrated virus was 2.2×1011TU/L. HGC-27 cells infected with the lentivirus, the expression level of BC002811 was enhanced significantly(P<0.05). MTS assay results showed that the proliferation ability of HGC-27 cells in the BC002811 group was increased as compared with the control group(P<0.05). Conclusion The BC002811 recombinant lentiviral vector was successfully constructed, which could stably transfect HGC-27 cells to yield sustained over-expression of BC002811 and promoted cell proliferation.