〔Abstract〕 Objective To investigate the effects of Transforming growth factor-β1(TGF-β1) on rabbit endometrial glandular epithelial cells to establish an epithelial-mesenchymal transition(EMT) model of endometrial epithelial cells. Methods Endometrial epithelial cells of New Zealand white rabbits were isolated and cultured, with different concentrations(0, 10, 60, 110 μg/L) of TGF-β1induced 24 h and 48 h.EMT model was established by screening the optimal concentration and time of induction of TGF-β1.Morphological changes of cells were observed,CCK-8 was used to detect cell proliferation inhibition, and Western-blot and immunohistochemistry were used to detect changes in vimentin(VIM) and E-cadherin(E-cadherin) expression. Results 60 μg/L TGF-β1inducing cell for 24 h significantly inhibited the proliferation of rabbit endometrial epithelial cells, changed cell morphology and decreased cell density to transform cells into mesenchymal morphology. Meanwhile, it down-regulated expression of E-cadherin protein and up-regulated expression of VIM protein. Conclusion The epithelial-mesenchymal transition model of rabbit endometrial epithelial cells can be successfully established with the concentration of 60 μg/L TGF-β1inducing cells for 24 h.