〔Abstract〕 Objective To analyze the effect and molecular mechanism of phellodendron amurense in the treatment of liver injury based on network pharmacology, and to verify the relevant prediction targets and the protective effect of phellodendron amurense extract-Phellodendron amurense polysaccharide on immune liver injury through mice. Methods TCMSP and Swiss target prediction databases were used to retrieve and screen phellodendron amurenses active components and action targets, and then obtain disease-related targets on GeneCards and OMIM websites, and take compounds and disease intersection targets for protein interaction. Analysis, GO biological function and KEGG signaling pathway enrichment analysis, followed by molecular docking of compounds and key target proteins, and finally established a mouse liver injury model induced by Daodou protein A(Con A) to explore the mechanism of phellodendron amurense extract in the treatment of liver injury. Results 37 active ingredients were screened. The key targets for their treatment were tumor necrosis factor α(TNF-α), serine/threonine protein kinase 1(AKT1), signal transduction and transcription activation factor 3(STAT3), epidermal growth factor receptor(EGFR) anditin. Enzyme 3(CASP3) and other enrichment analysis showed that phellodendron amurense might play a protective role in protecting the liver through molecular mechanisms such as positive regulation of MAPK cascade reaction, oxidative stress response and regulatory PI3K-Akt signaling pathway, lipid and atherosclerosis. Animal experiments had found that the gastric treatment of phellodendron amurense polysaccharide could improve the activity of superoxide dismutase(SOD) and catalase(CAT) in liver tissue, reduce the levels of serum alkaline phosphatase(ALP), aspartate aminotransferase(AST) and malonaldehyde(MDA) in liver tissue, and regulate serum inflammatory factor while the expression of intercitin(IL)-6, IL-1β, tumor necrosis factor α(TNF-α), activated the expression of transforming growth factor β1(TGF-β1), and reduced TNF-α mRNA expression in liver tissue. Conclusion Phellodendron amurense can intervene in lipid and atherosclerosis pathways by acting on targets such as TNF-α, AKT1, STAT3, EGFR and CASP3 to reduce oxidative stress and inflammatory reactions and achieve liver protection.