〔Abstract〕 Objective To investigate the expression of Member RAS Oncogene Family(RAB10) in pancreatic cancer(PAAD) and its effects on the proliferation, migration, invasion and apoptosis of SW1990 cells(human pancreatic cancer cells). Methods The expression ofRAB10mRNA in PAAD tissues wasanalyzed by the cancer gene database GEPIA(Gene Expression Profiling Interactive Analysis) and TCGA(The Cancer Genome Atlas). Cox regression analysis was used to detect relationship betweenRAB10mRNA expression and the prognosis of pancreatic cancer patients. We targeted small interfering RNA(RAB10-siRNA) targetingRAB10as the silence group, and constructed an overexpression plasmid(RAB10-OE) for overexpression ofRAB10as the overexpression group. The effects of silencing and overexpressionweredetected by Q-PCR; protein expression levelsweredetected by Western blot.EdUcellproliferation test, wound healing test, Transwelltestand flow cytometry test were used to determine the effects ofRAB10on the proliferation, migration, invasion and apoptosis of SW1990 pancreatic cancer cells. Results RAB10mRNA expression in PAAD tissues was higher than that innormal pancreatic tissues(P<0.05). The results of EdUcellproliferation testshowed that the proliferation rate of SW1990 cells in the RAB10-OE group was higher thanthat in the control group, and the proliferation rate of SW1990 cells in the RAB10-siRNA group was lower than that inthe control group(P<0.05). The results of the Transwell test and wound healing test showed that the invasion rate and mobility rate of RAB10-OE group were higher thanthose of the control group, and the mobility and invasion rate of RAB10-siRNA group were lower than those of the control group(P<0.05). The results of flow cytometry test showed that the apoptosis rate was lower in the RAB10-OE group than the control group, and the apoptosis rate in the RAB10-siRNA group was higher than the control group(P<0.05). The median survival time ofRAB10high expression group was significantly lower than that ofRAB10low expression group(P<0.05). Cox regression analysis showed that clinical grade, T stage, M stage andRAB10mRNA expression were related to survival and prognosis of pancreatic cancerpatients(P<0.05). Multivariate Cox regression analysis showed that the expression level ofRAB10mRNA was the independent risk factor affecting the prognosis of pancreatic cancer patients(P<0.05). Conclusion RAB10is highly expressed in PAAD tissues andRAB10can promote the proliferation of pancreatic cancer cells, accelerate the ability to invade and migrate, and inhibit the apoptosis of pancreatic cancer cells.RAB10is an independent risk factor for survival prognosis in patients with pancreatic cancer.