〔Abstract〕 Objective To investigate the impact of positive antinuclear antibody(ANA) and subsequent intervention therapy on the assisted reproductive technology outcomes among the patients experiencing recurrent implantation failure(RIF). Methods A retrospective study was conducted on 344 RIF patients. Based on ANA test results, the patients were divided into ANA-positive group(294 cases) and negative control group(50 cases). The ANA-positive group was further divided into a low titer group(214 cases) and a high titer group(80 cases). Comparative statistical analyses such as the Wilcoxon rank-sum test, Mann-Whitney U test, Kruskal-Wallis test and chi-square test, etc. were employed to evaluate differences in general clinical data, embryo-related parameters, and pregnancy outcomes between the positive and negative groups. The impact of ANA on the assisted reproductive outcomes of patients with recurrent implantation failure was analyzed, and the outcomes of ANA-positive patients after intervention therapy were also analyzed. Results Notably, the clinical pregnancy rates of patients in the ANA-positive low titer group and high titer group were significantly lower than those in the negative control group(P<0.001). Similarly, the rates of fertilization and cleavage of oocytes in ANA-positive patients were also significantly lower than those in the negative control group(P<0.05). For patients who did not achieve pregnancy after embryo transfer due to ANA positivity, immunomodulatory therapy significantly improved both the clinical pregnancy rate and cumulative clinical pregnancy rate(P<0.05). Conclusion Compared with the negative control group, the clinical pregnancy rates decrease in both ANA-positive low titer subgroup and high titer subgroup. However, clinical intervention therapy enhances the single-cycle clinical pregnancy and cumulative pregnancy rates among ANA-positive patients, indicating that ANA positivity is an important factor in RIF. Immunomodulatory therapy is an effective measure to improve recurrent implantation failure among ANA-positive patients.