〔Abstract〕 Objective To detect the low-affinity penicillin-binding protein drug resistance, pbp4 gene, and multi-locus sequence typing(MLST) of clinically isolated E. faecalis. Methods 78 clinical isolates of E. faecalis were collected, and their drug resistance was detected by automated instruments; the mutation of pbp4 gene mutation was analyzed by PCR amplification and MLST. Results 78 strains of E. faecalis were highly resistant to ciprofloxacin, levofloxacin, rifampicin, erythromycin, tetracycline and high concentration of gentamicin, and were resistant to penicillin and gentamicin. The ampicillin resistance rate was 10.3%, and no strains were found to be resistant to nitrofurantoin, vancomycin, teicoplanin and linezolid; 8 strains of 78 E. faecalis had amplified TEM genes, and all of them were resistant to penicillin and ampicillin resistance, with a positive rate of 10.3%; the allelic profiles and sequence types of 78 strains of E. faecalis which were divided into 16 sequence types, of which ST179 and ST16 were the most, with 21 and 21 strains, respectively. 20 strains, accounting for 26.9% and 25.6%, the rest were ST6 type 8 strains(10.3%), ST4 type 7 strains(9.0%), ST585 type 6 strains(7.7%), ST480 type 4 strains(5.1%), ST28 strains 3 strains(3.8%) of the ST type were detected, and only 1 strain was detected for the other ST types. The analysis of the relationship between ST types and drug resistance showed that E. faecalis with the same ST type had similar drug resistance profiles. Conclusion The resistance mechanism of E. faecalis to β-lactam antibiotics is mainly caused by the production of β-lactamase mediated by TEM gene, which is not necessarily related to the mutation of pbp4 gene. The isolates of E. faecalis are mainly CC16(including ST16 and ST179) clones and drug resistance is serious. It is necessary to guide clinical medication and strengthen nosocomial infection monitoring according to its characteristics.