〔Abstract〕 Objective To analyze interacting proteins of tropomodulin1(TMOD1) in Raw264.7 mouse monocyte macrophage line by mass spectrometry and GeneCards database. Methods Immunoprecipitation combined with mass spectrometry was used to find interacting proteins of TMOD1 after overexpress TMOD1 in Raw264.7 cells. GeneCards database was used to search for known genes for macrophage migration. Bioinformatics&Systems Biology was used to analyze correlation between known targets and mass spectrometry proteins to find common differentially expressed proteins(CO-DEPs). WoLF PSORT was used to predict subcellular localization of CO-DEPs. EggNOG databasewas used to analyze eukaryotic orthologous group(KOG) of CO-DEPs. DAVID database was used to analyze gene ontology(GO) enrichment kyoto encyclopedia of genes and genomes(KEGG) pathway of CO-DEPs. String database was used to analyze protein interaction network and CytoScape software drawing. Results There were 41 CO-DEPs in mass spectrometry and GeneCards database. Subcellular localization of CO-DEPs was mainly distributed in cytoplasm, nucleus and mitochondria. KOG notes were mainly O: post-translational modification, Z: cytoskeleton and J: translation. GO enrichment found that CO-DEPs was mainly involved in poly(A) RNA binding, protein folding and focal adhesion. KEGG was mainly enriched in arrhythmogenic right ventricular cardiomyopathy(ARVC) and tight junction. ACTB was a protein with large protein interaction. Conclusion The proteins interacting with TMOD1 in macrophages mainly include myosin heavy chain-9(MYH9), α-actinin 1(ACTN1) and β-actin(ACTB), etc, suggesting that TMOD1 is related to macrophages migrate.