〔Abstract〕 Objective To investigate the association between single nucleotide polymorphism(SNP) rs558614, rs9552315, rs7317471 and rs9509492 in large tumor suppressor kinase 2(LATS2) gene and the risk of colorectal cancer. Methods A total of 390 colorectal cancer patients and 413 healthy subjects were genotyped by Taqman method. The odds ratio(OR) and its 95%CIwere calculated by unconditional logistic regression, to estimate the associations between SNP rs558614, rs9552315, rs7317471, rs9509492 in LATS2 gene and the risk of colorectal cancer, rectal cancer, as well as colon cancer under codominant, dominant, recessive, overdominant, and log-additive genetic models. Haplotypes were constructed by haploview software 4.2. Results SNP rs558614, rs7317471, rs9552315 and rs9509492 in LATS2 gene were not associated with the risk of colorectal cancer, rectal cancer and colon cancer under codominant, dominant, recessive, overdominant, and log-additive genetic models. No haploid blocks were formed between the 4 SNPs. Conclusion SNP rs558614, rs7317471, rs9552315, rs9509492 in LATS2 gene may not play a major role in the development of colorectal cancer, rectal cancer and colon cancer.