〔Abstract〕 Objective To study the correlation between eukaryotic translation initiation complex 4 f(EIF4 F) and clinical prognosis of patients with hepatocellular carcinoma(HCC). Methods By following up the clinical data of 743 HCC patients in the specimen bank, 94 HCC tissue specimens with complete follow-up information were selected, and the clinical data were collected. The paired tissue specimens were made into tissue chip for immunohistochemical staining. Image J was used to analyze the optical density value of tissue chip staining, and R4.0.5 software was used to conduct nonparametric test analysis, draw KM curve, Cox regression analysis, and Nomogram statistical analysis on experimental data and follow-up data. Results Phosphorylation of 4 EBP1 was significantly activated in HCC tissues(P<0.001), and the activation Phosphorylation of 4 EBP1 was associated with the clinical prognosis of HCC patients,P=0.038. Conclusion The activation of 4 EBP1 phosphorylation in tumor tissue predicts shorter overall survival time in HCC patients.