〔Abstract〕 Objective To investigate the expression and prognosis of urokinase plasminogen activator(PLAU) and v-akt murine thymoma viral oncogene homolog 1(AKT1) in oral squamous cell carcinoma(OSCC) and normal tissues and the correlation of PLAU and AKT1 in OSCC tissues. Methods The expression levels of PLAU and AKT1 in 70 cases of oral squamous cell carcinoma and 50 cases of normal tissues were detected by immunohistochemical method, and the correlation between PLAU and AKT1 expression and clinicopathological features and prognosis as well as the correlation between PLAU and AKT1 expression in OSCC tissues was analyzed, and the results were further verified by bioinformatics database. Results The expression of PLAU and AKT1 in OSCC tissues was higher than that in normal tissues(P<0.05),Kaplan-Meier analysis showed that patients with low PLAU and AKT1 expression had longer survival time than those with high AKT1 expression(P<0.05),Spearman rank sum correlation test showed that there was a strong correlation between PLAU and AKT1 expression in OSCC tissues(r=0.357,P<0.05),GEPIA bioinformatics database analysis results are consistent with experimental results. Conclusion PLAU and AKT1 are highly expressed in OSCC tissues and are associated with poor prognosis of patients. There is a correlation between PLAU and AKT1 in OSCC tissues.