〔Abstract〕 Objective To investigate the effect of electroacupuncture(EA) treatment at pinch points(EX-B2) on motor function and Notch signaling pathway after spinal cord injury(SCI). Methods Seventy-two Sprague-Dawley(SD) male rats weighing(250±20) g were randomly divided into sham operation group, SCI, electroacupuncture group(SCI+EA) and acupuncture group(SCI+AP), with 18 rats in each group. A rat acute SCI model of T10 was established by the modified Allen′s method. The SCI+EA and SCI+AP groups received 15 minutes of electro acupuncture or acupuncture treatment per day. The motor function of the hind limbs of rats was assessed by Basso, Beattie and Bresnahan(BBB) scoring method, the pathological recovery changes of spinal cord tissues were observed by Hematoxylin-eosin(HE) staining, and the mRNA and protein expression levels ofHes 3,Notch 3andNotch 4were detected by real-time quantitative PCR and estern blot method, respectively, after 3, 7 and 14 days of intervention. The expression of glial fibrillary acidic protein(GFAP) was detected by immunohistochemistry after 14 days of intervention. Results Compared with the sham group, the BBB scores were reduced in rats after SCI surgery and there was significant hemorrhage, structural destruction and degeneration of spinal cord tissue(P<0.05), while the SCI+EA and SCI+AP groups were milder than the SCI group(P<0.05). The mRNA expression levels and protein expression levels ofHes 3,Notch 3andNotch 4as well as the expression levels of GFAP appeared significantly higher in the SCI group compared with the sham group(P<0.05), while the SCI+EA group appeared lower compared with the SCI group(P<0.05). Conclusion EA of EX-B2 can improve the locomotor function of rats with SCI, which may be related to inhibiting the expression of GFAP and the activation of Notch signaling pathway.