〔Abstract〕 Objective To investigate the mechanism of miRNAs in glycyrrhizic acid treatment of alcohol-induced liver injury in rats. Methods 45 male SD rats were randomly divided into glycyrrhizin group, model group and control group. The rats in glycyrrhizin group were given 56% liquor and glycyrrhizin, the rats in model group were given 56% liquor, and the rats in control group were given distilled water for 8 weeks.The blood was collected and the serum was separated by centrifugation to detect the levels of aspartate aminotransferase(AST) and alanine aminotransferase(ALT). RNA were extracted from liver tissues, miRNAs were detected by rat miRNA microarray, and their expression levels were analyzed. The miRNA target genes of differential miRNA were predicted. Gene Ontology(GO) and KEGG Pathway enrichment analysis were used to understand the function of differential miRNA, and the differential miRNA-mRNA-Pathway regulatory network was constructed using Cytoscape to further screen the regulatory key miRNA and key pathways. qRT-PCR was used to verify the expression of selected miRNA. Results Compared with the model group, the glycyrrhizin group could significantly improve the liver tissue lesions, and reduce the liver serum AST and ALT levels(P<0.05). Compared the microarray data of glycyrrhizin group and model group, a total of 13 differentially expressed miRNA were screened(P<0.05,fold change≥1.5), of which 10 were up-regulated and 3 were down-regulated. The GO classification annotation of differential miRNA target genes showed that differential miRNA were related to cell adhesion, antioxidant activity, metabolic process, biological process regulation, cell killing, immune system and other functions. The KEGG Pathway analysis of differential miRNA target genes showed that MAPK signaling pathway, mTOR signaling pathway, Ras signaling pathway, Hippo signaling pathway, PI3K-Akt signaling pathway, wnt signaling pathway, apoptosis and other signaling pathways might play an important regulatory role in the improvement of alcoholic liver injury by glycyrrhic acid. Conclusion This study established the miRNA expression profile of glycyrrhizin in the treatment of alcoholic liver injury in rats, suggested that miR-615, miR-107-3p and miR-292-5p might play an important role in the treatment of alcoholic liver injury by glycyrrhizin.