〔Abstract〕 Objective To investigate the effects of miR-130a-3p targeting Sirtuin7(Sirt7) on the migration, invasion and epithelial-mesenchymal transformation(EMT) of human trophoblast cells HTR-8/SVneo under hypoxic condition and its mechanism. Methods HTR-8/SVneo cells were studied as subjects, miR-130a-3p inhibitor and its negative control inhibitor-NC were transfected into the cells or combined with Sirt7 inhibitor 97491 intervention, treated with hypoxia(1% O2) for 48 h. qRT-PCR was used to detect the expression levels of miR-130a-3p and Sirt7 mRNA. Transwell assay was used to detect the ability of cells migration and invasion. The protein expression levels of Sirt7, HIF-1α, MMP-2, MMP-9, E-cadherin, N-cadherin and Vimentin were detected by Western blot. The targeting relationship between miR-130a-3p and Sirt7 was predicted and verified by TargetScan Human 7.1 online software and dual luciferase reporter assay. Results Hypoxia up-regulated the expression of miR-130a-3p in HTR-8/SVneo cells, down-regulated the expression of Sirt7 mRNA and protein. The low expression of miR-130a-3p increased the expression of Sirt7 mRNA, promoted the migration and invasion ability of cells, down-regulated the expression of HIF-1α and E-cadherin protein, and up-regulated the expression of Sirt7, MMP-2, MMP-9, N-cadherin and Vimentin protein. However, Combined treatment with Sirt7 inhibitor 97491 reversed the promotion effect of miR-130a-3p low expression on the migration, invasion and EMT of hypoxic cells. Conclusion miR-130a-3p is highly expressed in hypoxia-induced trophoblast cells. Inhibiting the expression of miR-130a-3p promotes the ability of migration, invasion and EMT of HTR-8/SVneo cells under hypoxic condition, which may be achieved by targeting Sirt7 to regulate HIF-1α.