〔Abstract〕 Objective To explore the general information, clinical features, imaging findings and outcome of patients with drug-induced cholestatic. Methods The clinical data of patients diagnosed as cholestatic drug-induced liver injury in recent 5 years were analyzed retrospectively, and followed up for at least 6 months. Results There were 105 patients with drug-induced cholestatic liver injury in the hospital, the median age was 55 years old(54.3% male),most of them had no specific clinical symptoms; there were nearly 80 kinds of drugs involved, the top three were traditional Chinese medicine(34.3%),antineoplastic drugs(22.9%) and antibiotics(10.5%).Among the 49 patients with magnetic resonance cholangiopancreatography(MRCP),7(14.3%) had sclerosing cholangitis-like changes; most of the patients were combined with multiple liver-protecting and enzyme-lowering drugs; there were 53 cured(50.5%),41 improved(39.0%),7 uncured(6.7%),and 4 died(3.8%) among 105 patients, and the total effective rate was 89.5%. Conclusion Drug-induced cholestatic liver injury mainly occurs in elderly patients, involving many kinds of drugs, mainly traditional Chinese medicine.Drugs can also cause changes similar to sclerosing cholangitis, so drug-induced liver injury(DILI) should be used as one of the differential diagnosis of cholestasis and abnormal cholangiography.Liver disease and the number of drugs have no effect on the prognosis of the patients, while low Roussel Uclaf causality assessment method(RUCAM) score and hemoglobin count, short hospitalization time and high white blood cell count are poor prognostic factors.